Aqueous solutions of cysteine oxidize to cystine (and by analogy, N-Acetyl-L-Cysteine to N,N'-Diacetyl-L-Cystine) upon exposure to air at neutral or alkaline pH. The pH of a solution of this product in water has not been measured. So if it is found that the pH is 7 or greater, the addition of a drop or two of dilute hydrochloric acid would be sufficient to stabilize that solution (pH 5, for example). Aqueous stock solutions are stable if frozen for up to 1 month at -20°C.
A9165
N-Acetyl-L-Cystein
≥99% (TLC), suitable for cell culture, BioReagent
N-Acetyl-L-cysteine
Synonym(e):
LNAC, NAC
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5 G
€ 25,10
25 G
€ 85,60
100 G
€ 289,00
Über diesen Artikel
Lineare Formel:
HSCH2CH(NHCOCH3)CO2H
CAS-Nummer:
Molekulargewicht:
163.19
NACRES:
NA.26
PubChem Substance ID:
UNSPSC Code:
12352209
EC Number:
210-498-3
MDL number:
Beilstein/REAXYS Number:
1724426
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Unterstützung erhaltenProduktname
N-Acetyl-L-Cystein, suitable for cell culture, BioReagent
product line
BioReagent
Quality Level
assay
≥99% (TLC)
form
powder
technique(s)
cell culture | mammalian: suitable
color
white to off-white
mp
106-108 °C (lit.)
solubility
H2O: 100 mg/mL (with heating)
storage temp.
2-8°C
SMILES string
CC(=O)N[C@@H](CS)C(O)=O
InChI
1S/C5H9NO3S/c1-3(7)6-4(2-10)5(8)9/h4,10H,2H2,1H3,(H,6,7)(H,8,9)/t4-/m0/s1
InChI key
PWKSKIMOESPYIA-BYPYZUCNSA-N
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1 of 4
Dieser Artikel | |||
|---|---|---|---|
| form powder | form powder | form powder | form solid |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable | technique(s) - |
| assay ≥99% (TLC) | assay ≥99% (TLC) | assay ≥99% (TLC) | assay - |
| product line BioReagent | product line - | product line BioXtra | product line - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-30°C |
| mp 106-108 °C (lit.) | mp 106-108 °C (lit.) | mp 106-108 °C (lit.) | mp 110.4 °C |
Application
N-Acetyl-L-Cystein wird als Komponente des Darm-Hauptkulturmediums für die Kultivierung von Darm-Stammzellen bei Mäusen wie auch als Komponente des Expansionsmediums verwendet.
Biochem/physiol Actions
Antioxidans und mukolytisches Agens. Erhöht den zellulären Pool an Radikalfängern. Apoptose verhindernde Wirkung in neuronalen Zellen und Apoptose induzierende Wirkung in glatten Muskelzellen wurde beobachtet. Inhibiert HIV Replikation. Fungiert möglicherweise als Substrat für mikrosomale Glutathion-Transferase.
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Beschreibung
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signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Lagerklasse
13 - Non Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Verwandter Inhalt
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| A9165-100G | 04061833401392 |
| A9165-25G | 04061833401408 |
| A9165-5G | 04061833401415 |
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is it necessary to adjust the ph if it is prepared in water?
1 answer-
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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Is this compound suitable for in vivo i.p. administration (mice)? Or is there an alternate formulation of the N-Acetyl-L-cysteine?
1 answer-
The product is not tested to determine its suitability for in vivo use on laboratory animals, nor is a product available that is known to be suitable for such use.
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Is this product sterile? If not, can I pass it through a 0.22 um filter unit?
1 answer-
This product is not supplied sterile. However, if the water used is ultrapure, cell culture grade, and prepared in a sterile environment, there is no need to filter the solution.
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Can aliquots be freeze-thawed more than once?
1 answer-
Product A9165 recommended storage is 2-8°C, Regrettably, information about stability after the material is reconstituted is unavailable. Generally speaking, avoid repeated freeze-thawed.
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Can aliquots be stored long term at -80 Celsius?
1 answer-
As mentioned in the previous Q&A, it is recommended to store the aliquoted solutions at -20 °C for up to 1 month. Stability testing at -80°C has not been performed. It is not known if lower storage temperatures will extend the stability of the solution.
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How can I make 1M N-acyteyl cysteine solution? Should I dilute in PBS or distilled water?
1 answer-
This product can be dissolved in H2O up to 100 mg/mL (with heating) . PBS can be used as well. Aliquot and store solutions at -20 deg. C up to 1 month.
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What is the molecular weight of product A9165, N-Acetyl-L-cysteine (NAC)?
1 answer-
As listed on the product detail page, the molecular weight is determined as 163.19
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My media turned yellow when I added N-acetyl cysteine (NAC), Product A9165. Why?
1 answer-
The pH of a 1% solution is 2.0-2.8. You may need to adjust the pH of the media with NaOH. Or, prior to addition to the media, you can initially dilute the NAC with PBS, and then add it to the culture media.
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