This product can be prediluted with 3-10 parts of cool water to aid in dispersion. Prediluted suspensions should be used immediately; they are not as stable as the emulsion as provided, and they should not be stored for later use.
A8011
Entschäumer C-Emulsion
aqueous-silicone emulsion
Synonym(e):
Antischaummittel-Emulsion
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250 ML
€ 170,00
500 ML
€ 296,00
Über diesen Artikel
NACRES:
NA.25
UNSPSC Code:
12161900
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Unterstützung erhaltenbiological source
synthetic
form
emulsion
contains
emulsifier
technique(s)
cell culture | hybridoma: suitable, microbiological culture: suitable
Quality Level
viscosity
400 cP
mp
0 °C (32 °F)
density
1.01 g/mL
application(s)
(Supplement in culture media)
1 of 4
Dieser Artikel | |||
|---|---|---|---|
| form emulsion | form emulsion | form emulsion (aqueous) | form emulsion (aqueous) |
| technique(s) cell culture | hybridoma: suitable, microbiological culture: suitable | technique(s) cell culture | hybridoma: suitable, microbiological culture: suitable | technique(s) cell culture | hybridoma: suitable, microbiological culture: suitable | technique(s) cell culture | hybridoma: suitable |
| biological source synthetic | biological source - | biological source - | biological source - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| mp 0 °C (32 °F) | mp - | mp - | mp - |
| contains emulsifier | contains emulsifier (different from those present in Antifoam A emulsion) | contains - | contains - |
General description
Entschäumer C-Emulsion ist eine wässrige Emulsion, die 30 % aktives Silikon enthält. Diese Entschäumer-Emulsion auf Silikonbasis[1] besteht aus nichtionischen Emulgatoren, die sich von denen in Entschäumer-Emulsionen B und Y-30 unterscheiden. Entschäumer C-Emulsion kann mit 3–10 Teilen kaltem Wasser vorverdünnt werden, um die Dispersion zu unterstützen. Vorverdünnte Suspensionen sollten umgehend verwendet werden. Entschäumer C-Emulsion ist in der Regel zu 1–10 ppm wirksam. Die Flusseigenschaften von Entschäumer C-Emulsion sorgen dafür, dass sie nach Bedarf in eine Fermenter-Anlage mit ausreichender Bewegung zum Dispergieren des Entschäumers gepumpt werden kann. Entschäumer C-Emulsion kann zum Regulieren des Schäumens im Gasraum von Säugetierzellkultur-Bioreaktoren in einer Konzentration von bis zu 30 ppm eingesetzt werden.[1]
Application
Entschäumer C-Emulsion wurde wie folgt verwendet:
- als Zusatz in RO-H2O und als chemisch definiertes Kulturmedium zum Bestimmen des volumenbezogenen Stoffübergangskoeffizienten (kLa) von Säugetierzellkulturen[2]
- als Komponente von Nährmedium[2]
- als dampfsterilisierter Entschäumer in einem Biocontroller für pH-stat-Fed-Batch-Kulturen[3]
- als Zusatz in Fed-Batch-Medium zum Nachahmen der typischen Bedingungen einer Kultur auf großem Maßstab[4]
Features and Benefits
- Minimiert Kompatibilitätsprobleme mit biologischen Systemen
- Hochwirksamer Entschäumer bei niedrigen Konzentrationen
- Hochwirksamer und vielseitiger Silikon-Entschäumer
Other Notes
30%ige wässrige Emulsion eines Polydimethylsiloxan-Konzentrats.
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signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Lagerklasse
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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Bernhard Sissolak et al.
Biotechnology journal, 14(7), e1800714-e1800714 (2019-04-16)
Frequently measured mammalian cell culture process indicators include viability and total cell concentration (TCC). Cell lysis, an additional important process characteristic that substantially contributes to the overall product purity profiles, is often not addressed in detail. In the present study
Gang Fu et al.
Biotechnology and applied biochemistry, 67(5), 812-818 (2019-10-08)
d-Psicose 3-epimerase is an enzyme that catalyzes the synthesis of d-psicose from d-fructose. We cloned the d-psicose 3-epimerase from Ruminococcus sp. (RDPE) and expressed it in Bacillus subtilis A311. By a two-step pH regulation of segmented fermentation, we significantly improved
Characterizing the Effects of Antifoam C Emulsion on Oxygen Mass Transfer within the BIOne Drilled-Hole Sparger and Microsparger Single-Use Bioreactor Systems
McAndrew J and Kauffman G
American Pharmacy null
Mervyn W O Liew et al.
Journal of biotechnology, 150(2), 224-231 (2010-08-28)
This study demonstrates the feasibility of large-scale production of murine polyomavirus VP1 protein in recombinant Escherichia coli as pentamers which are able to subsequently self-assemble in vitro into virus-like particles (VLPs). High-cell-density pH-stat fed-batch cultivation was employed to produce glutathione-S-transferase
Magdalena Pappenreiter et al.
Frontiers in bioengineering and biotechnology, 7, 195-195 (2019-09-10)
In aerobic cell cultivation processes, dissolved oxygen is a key process parameter, and an optimal oxygen supply has to be ensured for proper process performance. To achieve optimal growth and/or product formation, the rate of oxygen transfer has to be
Verwandter Inhalt
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After dilution, how long is the product stable for? If I were to use a 1:10 or 2% solution can I keep it at RT for 14 days next to the bioreactor?
1 answer-
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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What is the particle size range of the silicone emulsion particles in this anti-foam. Thank you!
1 answer-
The particle size of this product is not determined. However, silicon-based antifoam formulations have a general particle size range of 10um - 40um.
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Will there be a problem if I add 100ppm of antifoam when cultivating bacteria?
1 answer-
This product is typically effective at 1-10 ppm. Therefore, there is no guarantee how 100 ppm will perform for bacteria cultivation.
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Can the antifoam be autoclaved pure? When diluted (e.g. to 0.5% or 0.1%)?
1 answer-
Yes, this product may be autoclaved. Autoclaving the pure product may result in phase separation and might require remixing the emulsion.
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