Potassium buffer is not required. For assay purposes, this product is reconstituted in potassium phosphate buffer, pH 6.0, at 0.2 mg/ml, Solutions may also be prepared 40 mM Tris HCL, pH 7.5, containing 50 mM NaCl and 50% glycerol. This stock may be stored for extended periods at –20 °C. Other sources indicate that this enzyme may also be prepared in water containing 0.01% BSA and TBE buffer. These options have not been validated. Please see the link below to review a publication that may be of interest:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2817930/
A6306
Agarase aus Pseudomonas atlantica
lyophilized powder, ≥5,000 units/mg protein (Lowry)
Synonym(e):
Agarose-3-glycanohydrolase
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1000 UNITS
€ 383,00
5000 UNITS
€ 938,00
€ 383,00
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Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
eCl@ss:
42040102
NACRES:
NA.26
MDL number:
Specific activity:
≥5,000 units/mg protein (Lowry)
Biological source:
bacterial (Pseudomonas atlantica)
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Unterstützung erhaltenbiological source
bacterial (Pseudomonas atlantica)
Quality Level
form
lyophilized powder
specific activity
≥5,000 units/mg protein (Lowry)
storage temp.
2-8°C
Verwandte Kategorien
Physical form
Enthält Phosphat-Puffersalze. Kann Rinderserumalbumin zur Standardisierung des Proteingehalts enthalten.
Other Notes
1 U bildet 1.0 μg reduzierender Zucker (gemessen als D-Galactose) aus Agar pro Minute bei pH 6.0 und 40 °C.
Für weitere Informationen siehe enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Yuji Hatada et al.
Marine biotechnology (New York, N.Y.), 13(3), 411-422 (2010-08-06)
A gene of unknown function from the genome of the agar-degrading deep-sea bacterium Microbulbifer thermotolerans JAMB-A94(T) was functionally identified as a ι-carrageenase gene. This gene, designated as cgiA, is located together with two β-agarase genes, agaA and agaO in a
The effects of short- and long-term freezing on Porphyra umbilicalis Kutzing (Bangiales, Rhodophyta) blade viability
Lindsay Green
Journal of Embryology and Experimental Morphology (2014)
Uyangaa Temuujin et al.
Applied microbiology and biotechnology, 92(4), 749-759 (2011-06-10)
The DagA product of Streptomyces coelicolor is an agarase with a primary translation product (35 kDa) of 309 amino acids, including a 30-amino acid signal peptide. Although dagA expression in Streptomyces lividans under the control of its own set of
Agarolytic bacterium Persicobacter sp. CCB-QB2 exhibited
a diauxic growth involving galactose utilization pathway
a diauxic growth involving galactose utilization pathway
Go Furusawa
Microbiology (2016)
Effects of temperature, light level, photoperiod, and ammonium concentration on Pyropia leucosticta (Bangiales, Rhodophyta) from the Northwest Atlantic
Lindsay Gree
Journal of Applied Phycology (2015)
Verwandter Inhalt
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A6306-1KU | 04061833382042 |
| A6306-5KU | 04061833382059 |
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Will this enzyme work in water or PBS, or must it be a potassium buffer (for which not further details are provided in the spec sheet)?
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