A5691

Monoklonaler Anti-Aktin-Antikörper , α-Glattmuskel

clone 1A4, purified from hybridoma cell culture

• Synonym(e): SMA
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: alkaline phosphatase conjugate
• Clone: 1A4, monoclonal
• Application: ELISA, IHC (p), WB
• Citations: 77

Eigenschaften

• biological source: mouse
• Quality Segment: 200
• conjugate: alkaline phosphatase conjugate
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 1A4, monoclonal
• form: buffered aqueous glycerol solution
• mol wt: antigen ~42 kDa
• species reactivity: human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake
• technique(s): ELISA: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using human tonsil or appendix sections, western blot: 1:100 using chicken gizzard extract/ Mouse heart extract
• isotype: IgG2a
• UniProt accession no.: P62736
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: mouse ... Acta2(11475) ; rat ... Acta2(81633)

Beschreibung

General description

Der Antikörper (auch als Anti-α-Sm-1 bekannt) ist spezifisch für die α-Isoform des Glattmuskelaktins. Er reagiert spezifisch mit α-Glattmuskelaktin in Immunblotting-Assays und markiert glatte Muskelzellen in gefrorenen oder Formalin-fixierten Paraffin-eingebetteten Gewebeschnitten.

Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three α actins (skeletal, cardiac, smooth), one β actin (β-non-muscle) and two γ actins (γ smooth muscle and γ non-muscle).

Immunogen

N-terminales synthetisches Decapeptid von α-Glattmuskelaktin.

Application

Glatte Muskelzellen boviner Aorten werden unter Verwendung des monoklonalen Anti-ACTA2-Antikörpers immunzytochemisch untersucht. Die Zellen werden zunächst auf Deckgläsern gezüchtet und 10 Minuten bei 4 Grad in 50 % Aceton/EtOH fixiert.

Paraffinschnitte von Rattenhoden-Gewebetransplantaten werden immunhistochemisch mit monoklonalen Maus-Antikörpern gegen Glattmuskelaktin gefärbt.
Zur Identifizierung von Myofibroblasten wird X-Gal-gefärbtes Maus-Herzgewebe unter Anwendung des monoklonalen Maus-Primärantikörpers gegen Glattmuskelaktin immunhistochemisch untersucht.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Immunocytochemistry was performed on kertocytes fixed in 1% PFA and incubated with mouse monoclonal anti-smooth muscle actin (14A) at a 1:100 dilution. [1]

Mouse monoclonal anti-actin, α−smooth muscle-alkaline phosphatase antibody has been used for immunohistochemical application in mouse and human tissues.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl₂, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide as a preservative.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 2
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)