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15205

Blasticidin S -hydrochlorid

Blasticidine S hydrochloride

Synonym(e):

Blasticidin S

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25 MG

€ 252,00

100 MG

€ 752,00

€ 252,00


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Über diesen Artikel

Empirische Formel (Hill-System):
C17H26N8O5 · HCl
CAS-Nummer:
Molekulargewicht:
458.90
NACRES:
NA.76
PubChem Substance ID:
UNSPSC Code:
51102829
MDL number:

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InChI

1S/C17H26N8O5.ClH/c1-24(16(20)21)6-4-9(18)8-12(26)22-10-2-3-13(30-14(10)15(27)28)25-7-5-11(19)23-17(25)29;/h2-3,5,7,9-10,13-14H,4,6,8,18H2,1H3,(H3,20,21)(H,22,26)(H,27,28)(H2,19,23,29);1H/t9-,10+,13-,14+;/m1./s1

SMILES string

Cl[H].CN(CC[C@@H](N)CC(=O)N[C@H]1C=C[C@@H](O[C@@H]1C(O)=O)N2C=CC(N)=NC2=O)C(N)=N

InChI key

YQXYQOXRCNEATG-ZAYJLJTISA-N

form

powder

color

white to off-white

antibiotic activity spectrum

fungi

application(s)

agriculture
environmental

mode of action

protein synthesis | interferes

shipped in

dry ice

storage temp.

−20°C

Quality Level

solubility

water: soluble, clear, colorless to faintly yellow

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Dieser Artikel
203350H9773D3447
antibiotic activity spectrum

fungi

antibiotic activity spectrum

-

antibiotic activity spectrum

fungi

antibiotic activity spectrum

Gram-negative bacteria, Gram-positive bacteria, mycoplasma, parasites

mode of action

protein synthesis | interferes

mode of action

-

mode of action

protein synthesis | interferes

mode of action

protein synthesis | interferes

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

application(s)

agriculture
environmental

application(s)

-

application(s)

agriculture

application(s)

-

form

powder

form

powder

form

lyophilized powder

form

powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

General description

Blasticidin S gehört zu der Aminoacylnukleosidklasse der Antibiotika. Es hemmt die Proteinsynthese in Bakterien und Eukaryoten.[1] Es weist außerdem fungizide Eigenschaften auf und verhindert Reisbräune.[2]
Chemische Struktur: Nukleosid

Application

Blasticidin S wird als Selektionsmittel für umgewandelte Zellen, die die Resistenzgene bls, bsr oder BSD enthalten, verwendet. Blasticidin S wird verwendet, um HEK293-T-Zellen mit TLR-2-Konstrukten und HEK-D5-Zellen auszuwählen[3][4]. Es wird außerdem zur Untersuchung der Proteinsynthese auf Ebene der Peptidbindungsbildung verwendet.

Biochem/physiol Actions

Blasticidin S hemmt die Hydrolyse von Peptidyl-tRNA, eingeleitet durch Freisetzungsfaktoren. Es verbessert die Bindung von tRNA an die große Untereinheit von Ribosomen, und hemmt in gewissem Maß die Bildung von Peptidbindungen.[1]

Packaging

In bodenlosen Glasflaschen. Der Inhalt befindet sich innerhalb eines eingeschobenen Schmelzkegels.

Other Notes

Behälter dicht verschlossen an einem trockenen und gut belüfteten Ort aufbewahren. An einem trockenen Ort aufbewahren. Lagerklasse (TRGS 510): Nicht entzündlich, akut toxisch, Kat. 1 und 2 / sehr toxische, gefährliche Materialien

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pictograms

Skull and crossbones

signalword

Danger

hcodes

Hazard Classifications

Acute Tox. 1 Oral

Lagerklasse

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Egor Svidritskiy et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(30), 12283-12288 (2013-07-05)
The antibiotic blasticidin S (BlaS) is a potent inhibitor of protein synthesis in bacteria and eukaryotes. We have determined a 3.4-Å crystal structure of BlaS bound to a 70S⋅tRNA ribosome complex and performed biochemical and single-molecule FRET experiments to determine
Rohini Garg et al.
Journal of immunology (Baltimore, Md. : 1950), 184(11), 5980-5987 (2010-05-05)
Vi capsular polysaccharide is a major virulence determinant of the human typhoid- causing pathogen Salmonella typhi; it is absent in nontyphoidal Salmonella serovars. We show in this study that through its specific interaction with the membrane recognition complex containing the
Elizabeth J Meredith et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(36), 13485-13490 (2006-08-30)
Human B lymphocytes and derived lines from a spectrum of B cell malignancy were studied for expression of dopaminergic pathway components and for their cytostatic response to the catecholamine and related, potentially therapeutic compounds. Proliferating normal lymphocytes and dividing malignant
James M Burke et al.
Molecular cell, 75(6), 1203-1217 (2019-09-09)
In response to foreign and endogenous double-stranded RNA (dsRNA), protein kinase R (PKR) and ribonuclease L (RNase L) reprogram translation in mammalian cells. PKR inhibits translation initiation through eIF2α phosphorylation, which triggers stress granule (SG) formation and promotes translation of
Zhihua Chen et al.
Development (Cambridge, England), 147(18) (2020-09-09)
The central nervous system (CNS) contains a complex network of blood vessels that promote normal tissue development and physiology. Abnormal control of blood vessel morphogenesis and maturation is linked to the pathogenesis of various neurodevelopmental diseases. The CNS-specific genes that

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Questions

1–5 of 5 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. How should aqueous solutions of Product 15205, Blasticidine S hydrochloride, be stored?

    1 answer
    1. Aqueous solutions of Blasticidine S hydrochloride should be stored in aliquots at -20°C.  Aqueous solutions at a pH °C or for 6 to 8 weeks at -20°C.  Avoid alkaline pH, and do not subject solutions to freeze-thaw cycles.

      Helpful?

  5. What is Product 15205, Blasticidine S hydrochloride, soluble in?

    1 answer
    1. Blasticidine S hydrochloride should be soluble in water at a concentration of 5 - 10 mg/mL. It is also soluble in acetic acid (concentration not reported).

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