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mol wt
Mw 1030 g/mol
solubility
DMF: 0.25 mg/mL, clear
fluorescence
λex 634 nm; λem 654 nm±10 nm in PBS, pH 7.4
suitability
suitable for fluorescence
storage temp.
−20°C
Quality Level
1 of 4
Dieser Artikel | |||
|---|---|---|---|
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence | suitability - |
| fluorescence λex 634 nm; λem 654 nm±10 nm in PBS, pH 7.4 | fluorescence λex 633, 635 or 640 nm (Flow cytometry laser line), λex 633, 635 or 640 nm (Microscopy laser line), λex 642 nm; λem 662 nm | fluorescence - | fluorescence λex 635 nm; λem 655 nm±10 nm in PBS, pH 7.4 |
| solubility DMF: 0.25 mg/mL, clear | solubility DMSO: soluble, H2O: soluble | solubility - | solubility DMF: 0.25 mg/mL, clear |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| mol wt Mw 1030 g/mol | mol wt ~832 g/mol (free acid) | mol wt - | mol wt - |
General description
Abberior STAR 635P can substitute Atto™ 647N, AlexaFluor 647, or Cy5. It can be excited with diode lasers (635 nm, 650 nm) or with the 647 nm line of a Krypton laser. For STED, a depletion wavelength of 750 - 780 nm is recommended. Please see reference1 for detailled characteristics.
Best results are obtained with freshly prepared samples.
Abberior STAR 635P particularly well suits the Leica TCS STED system and delivers high-resolution STED images with unmatched quality, e.g. extremely low/no background. The dye serves as an ideal partner for several 2-color STED.
Key Features
- First phosphorylated dye
- Unmatched, background free STED imaging contrast
- Verified in the Leica STED system
Absorption Maximum, λmax: 638 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 120′000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.21 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.40 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 651 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 750 - 780 nm
Fluorescence Quantum Yield, η: 0.90 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 3.3 ns (PBS, pH 7.4)
Application
Other Notes
Legal Information
Ähnliches Produkt
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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