E1903

Ethidium-Homodimer I -Lösung

≥95% purity (HPLC), liquid

• Synonym(e): EtDi, EthD-1, Phenanthridinium, 5,5′-[1,2-ethandiylbis-(imino-3,1-propandiyl)]-bis-(3, 8-diamino-6-phenyl)-,dichlorid,dihydrochlorid
• Empirische Formel (Hill-System): C₄₆H₅₀Cl₄N₈
• Molekulargewicht: 856.75
• NACRES: NA.47
• PubChem Substance ID: 24894444
• UNSPSC Code: 12171500
• EC Number: 200-664-3
• MDL number: MFCD00210948

Eigenschaften

• Produktname: Ethidium-Homodimer I -Lösung, ≥95% (Run on Ethidium Homodimer before dissolution in DMSO, TLC), 2 mM in DMSO
• Quality Segment: 100
• assay: ≥95% (Run on Ethidium Homodimer before dissolution in DMSO, TLC)
• form: liquid
• concentration: 2 mM in DMSO
• technique(s): titration: suitable
• color: red
• ε (extinction coefficient): ≥9000 at 490-496 nm in water
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: −20°C
• SMILES string: Cl.Cl.[Cl-].[Cl-].Nc1ccc2c(c1)c(-c3ccccc3)[n+](CCCNCCNCCC[n+]4c(-c5ccccc5)c6cc(N)ccc6c7ccc(N)cc47)c8cc(N)ccc28
• InChI: 1S/C46H46N8.4ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32;;;;/h1-6,9-20,27-30,49-52H,7-8,21-26,47-48H2;4*1H
• InChI key: ZKAJUKABUUJRLB-UHFFFAOYSA-N

Beschreibung

Application

Färbt ssDNA, dsDNA, RNA, Oligonucleotide und Triplex-DNA-Strukturen.

Für DNS-Detektion in Lösung.

Ethidium homodimer I solution has been used to check membrane integrity in sperms.

Biochem/physiol Actions

Ein Molekül EthD-1 bindet nicht-selektiv vier Basenpaare in dsDNA.

Ethidium homodimer is a red fluorescent dye. It cannot cross an intact plasma membrane, but can easily pass through a damaged plasma membrane. Ethidium homodimer is used to check membrane integrity.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: 185.0 °F - closed cup
• flash_point_c: 85 °C - closed cup
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)