67309

Kovac-Reagenz für Indole

suitable for microbiology

• Synonym(e): 4-Dimethylamino-benzaldehyd -Lösung
• Empirische Formel (Hill-System): C₉H₁₁NO
• CAS-Nummer: 100-10-7
• Molekulargewicht: 149.19
• UNSPSC Code: 41171621
• NACRES: NA.85
• PubChem Substance ID: 329760541
• MDL number: MFCD00003381
• Beilstein/REAXYS Number: 4132845

Eigenschaften

• agency: according to ISO 16654:2001
• Quality Segment: 200
• product line: BioChemika
• shelf life: limited shelf life, expiry date on the label
• composition: 4-(dimethlyamino)benzaldehyde, 50 g/L , hydrochloric acid, 240 g/L , isoamylic alcohol, 710 g/L
• technique(s): microbe id | specific enzyme detection: suitable
• application(s): agriculture; clinical testing; environmental; food and beverages, microbiology
• storage temp.: 2-8°C
• suitability: Escherichia coli, coliforms
• SMILES string: [H]C(=O)c1ccc(cc1)N(C)C
• InChI: 1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3
• InChI key: BGNGWHSBYQYVRX-UHFFFAOYSA-N

Beschreibung

General description

Kovac′s reagent is prepared by mixing p-dimethylaminobenzaldehyde, isoamyl alcohol and concentrated hydrochloric acid. For identification of an organism, the formation of Indole from a tryptophan substrate is a useful diagnostic tool. Indole production is a crucial test in identification of Escherichia coli. After incubation, adding the reagent will help in determination if Indole that has been produced. The reagent turns red when reacts with Indole.

Application

Kovac′s reagent may be used for spot test for determination of indole by saturating filter paper with the reagent. It may also be used for initial spot test for determination of indole followed by quantification by spectrophotometric assay and HPLC-UV/Vis-MS/MS methods.

In Anwesenheit von Sauerstoff können ein paar Bakterien, z.B. E. coli, Tryptophan in Indol und alpha-Aminopropionsäure aufspalten. Dieses Reagenz dient zur Detektion von Indol und zur Identifizierung von indol-positiven und indol-negativen Mikroorganismen.

Sicherheitshinweise

• target_organs: Respiratory system
• pictograms: GHS02,GHS05,GHS07
• signalword: Danger
• hcodes: H226,H290,H314,H317,H332,H335
• pcodes: P210 - P233 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
• Lagerklasse: 3 - Flammable liquids
• flash_point_f: 109.4 °F
• flash_point_c: 43 °C
• ppe: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter