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Merck

11758241001

Roche

β-Gal Reporter Gene Assay, chemiluminescent

sufficient for 500 assays (microplate), sufficient for 250 assays (tubes)

Synonym(e):

galactosidase

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

UNSPSC-Code:
23201100

Verwendung

sufficient for 250 assays (tubes)
sufficient for 500 assays (microplate)

Hersteller/Markenname

Roche

Lagertemp.

2-8°C

Allgemeine Beschreibung

The β-Gal Reporter Gene Assay is designed for specifically measuring bacterial β-galactosidase activity. To achieve this, the enzyme reaction is conducted at a pH that is optimized for bacterial β-Gal, but allows no significant endogenous eukaryotic β-galactosidase activity. However, if very high endogenous β-galactosidase activity is found (e.g., with hepatic cells), heat treatment can be performed to ensure the specific determination of the amount of bacterial β-galactosidase that is encoded by the transfected plasmid.

Anwendung

The β-Gal Reporter Gene Assay, chemiluminescent, is used to quantitatively measure β-Gal expression in eukaryotic cells that are transfected with a plasmid bearing the β-Gal-encoding lacZ reporter gene.

Leistungsmerkmale und Vorteile

  • Sensitive: Chemiluminescence technology allows detection of approximately 20 fg β-galactosidase in cell extracts
  • High dynamic measuring range: Linear range over four to five orders of magnitude
  • Constant light emission: The assay produces a long-lasting light emission instead of a short-peak kinetic
  • Safe: No radioactive isotopes are used
  • Fast: Approximately 1.5 to 2.5 hours elapse from start to finish
  • Convenient: Kit contains all reagents needed, including lysis buffer, protease inhibitors, and a positive control

Verpackung

1 kit containing 7 components.

Angaben zur Herstellung

Storage conditions (working solution): Substrate Reagent (solution 1) is stable at least for 24 h at 2 to 8 °C.
Initation Reagent (solution 2) is stable for at least 4 weeks when stored at 2 to 8 °C.
Lysis Reagent, 1x (solution 3) Lysis reagent without protease inhibitors is stable at
-15 to -25 °C or for 3 months at 2 to 8 °C. Lysis reagent containing protease inhibitors is stable for one week when stored frozen at -15 to -25 °C.
Positive Control (solution 4) The reconstituted enzyme solution is stable for 1 week at 2 to 8 °C. When stored frozen in aliquots at -15 to -25 °C the enzyme is stable for 3 months. Avoid multiple freezing and thawing.

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Nur Kit-Komponenten

Produkt-Nr.
Beschreibung

  • β-Gal Substrate, Galacton Plus 100x concentrated

  • Assay Buffer

  • Enhancer

  • Initiation Solution

  • Protease Inhibitors

  • Lysis Buffer 5x concentrated

  • Positive Control, β-galactosidase (E. coli), lyophilizate

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Zielorgane

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Lagerklassenschlüssel

3 - Flammable liquids

WGK

WGK 2


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