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SLM-241

DMEM - high glucose

Complete medium, with ʟ-glutamine and sodium pyruvate and 10% FBS, liquid, 0.2 μm filtered, suitable for cell culture

Synonym(e):

DME, DMEM with 10% FBS, Dulbecco′s Modified Eagle′s Medium - high glucose

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Ihnen/SKUVerfügbarkeitPreis
500 mL
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€ 201,00

Über diesen Artikel

UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.75

€ 201,00

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Produktname

DMEM Complete Medium, with 2mM L-Glut, Sodium Pyruvate, and 10% FBS, contains DMEM high glucose medium with 2mM L-glut, sodium pyruvate, and 10% U.S. origin FBS

Quality Segment

300

form

liquid

technique(s)

cell culture | mammalian: suitable

General description

Listen to an Audio Overview
Dulbecco′s Modified Eagle (DMEM) Complete Medium contains DMEM high glucose medium with 2mM L-glut, sodium pyruvate, and 10% U.S. origin FBS. DMEM Complete Medium is a convenient 0.2 μm filtered solution for the culture of primary mammalian cells including mouse and chicken cells, and a variety of normal and transformed cells.

Application

DMEM Complete Medium, with 2mM L-Glut, Sodium Pyruvate, and 10% FBS has been used:
  • to culture human gingival fibroblasts
  • to culture mouse fibroblast cells
  • as an overlay medium for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) plaque assay

Analysis Note

pH= 7.2-7.5
Osmolality= 320-346
Endotoxin= ≤ 2EU/mL
Mycoplasma= Negative
Sterility Tested= Negatvie for Growth
Appearance= Orange/ Red Clear Liquid
Cell Line Tested= HT-22 Human Neuronal Cells

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Lagerklasse

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Analysenzertifikate (COA)

Lot/Batch Number

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Questions

1–4 of 4 Questions  

  1. What is the shelf life from date of receipt when stored properly?

    1 answer

    1. The shelf life of this material is lot-specific. When stored as recommended (at -20 °C), the shelf life is valid up to the expiration date provided on the product label and Certificate of Analysis.

      Please see the link below to access a lot-specific Certificate:
      https://www.sigmaaldrich.com/product/mm/slm241#product-documentation

      Helpful?

  2. What is the composition of this product?

    1 answer

    1. The product is a comprehensive DMEM formulation that includes high glucose with 2mM L-glutamine, sodium pyruvate, and 10% FBS.

      Helpful?

  3. Can you provide the formulation for this media?

    1 answer

    1. The formulation of this media is proprietary. The following is known:

      Glucose: 4.5g/L (high Glucose Formulation)

      If you need to determine the presence or absence of a particular component or its concentration, please contact Technical Service with justification. For example, if the knowledge will support a specific downstream assay, we will then request this information. Please note that we cannot guarantee that this will be provided.

      Helpful?

  4. What is the formulation information available as compared to other DMEMs?

    1 answer

    1. This product is considered a complete DMEM formulation containing high glucose with 2mM L-glutamine, sodium pyruvate, and 10% FBS. Please see the link below to review and compare other classical DMEM formulations: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/380/910/d0572for.pdf

      Helpful?

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