The concentration of this antibody is 1 mg/mL. The starting dilution factor is recommended at 1:2000 (0.5 µg/mL) for immunoblotting and 1:200 (5 µg/mL) for immunocytochemistry applications.
PC408
Anti-Green Fluorescent Protein (26-39) Rabbit pAb
liquid, Calbiochem®
Synonym(e):
Anti-GFP
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100 μL
€ 678,00
€ 678,00
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Für Ihr Target ist ein rekombinanter, konservierungsmittelfreier Antikörper verfügbar. Probieren Sie ZRB1097
Über diesen Artikel
NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
(GFP and some GFP fusions)
Application:
—
Citations:
3
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Unterstützung erhaltenform
liquid
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
contains
≤0.1% sodium azide as preservative
species reactivity
(GFP and some GFP fusions)
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (1:2000)
Immunocytochemistry (1:200))
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
1 of 4
Dieser Artikel | SAB4200681 | SAB4701015 | AB3080 |
|---|---|---|---|
| biological source rabbit | biological source mouse | biological source rabbit | biological source rabbit |
| Quality Level 100 | Quality Level 200 | Quality Level - | Quality Level 100 |
| antibody form affinity isolated antibody | antibody form purified antibody | antibody form purified immunoglobulin | antibody form affinity purified immunoglobulin |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. - |
| clone polyclonal | clone GFP-20, monoclonal | clone - | clone polyclonal |
| form liquid | form buffered aqueous solution | form buffered aqueous solution | form - |
General description
Recognizes the ~28 kDa GFP and some GFP fusion proteins.
Anti-Green Fluorescent Protein (26-39), rabbit polyclonal, recognizes the ~28 kDa GFP and some GFP fusion proteins. It is validated for use in Western blotting and immunocytochemistry.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~28 kDa GFP and some GFP fusion proteins.
Immunogen
a synthetic peptide corresponding to amino acids 26-39 of Aequorea victoria wild-type green fluorescent protein
Aequora victoria
Application
Immunoblotting (1:2000)
Immunocytochemistry (1:200)
Immunocytochemistry (1:200)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, 1 mg/ml BSA.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Cubitt, A.B., et al. 1995. Trends Biochem. Sci.20, 448.
This sequence is completely conserved between GFP and the modified and enhanced Blue, Green, and Yellow FP variants. This antibody has been successfully used in immunoblotting and immunocytochemistry protocols to detect GFP and fusion proteins. It detects a ~28 kDa protein that corresponds to GFP in immunoblots of protein extract from cells transfected with, and overexpressing, GFP isoforms. Antibody should be titrated for optimal results in individual sytems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Produkt-Nr.
Beschreibung
Preisangaben
Lagerklasse
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Yan Zhang et al.
Nature, 615(7954), 884-891 (2023-03-17)
Calcium imaging with protein-based indicators1,2 is widely used to follow neural activity in intact nervous systems, but current protein sensors report neural activity at timescales much slower than electrical signalling and are limited by trade-offs between sensitivity and kinetics. Here
Kathryn A Tworkowski et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(16), 6103-6108 (2008-04-17)
Adenovirus E1A drives oncogenesis by targeting key regulatory pathways that are critical for cellular growth control. The interaction of E1A with p400 is essential for many E1A activities, but the downstream target of this interaction is unknown. Here, we present
Chih-Hao Lu et al.
Nature communications, 13(1), 3093-3093 (2022-06-03)
The glycocalyx is a shell of heavily glycosylated proteins and lipids distributed on the cell surface of nearly all cell types. Recently, it has been found that bulky transmembrane glycoproteins such as MUC1 can modulate membrane shape by inducing membrane
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Bonjour, Quelle est la concentration d'utilisation préconisée pour cet anticorps? Merci
1 answer-
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