MABF521

Anti-CD11c Antibody (human), APC, clone 3.9

clone 3.9, from mouse

• Synonym(e): Integrin alpha-X, CD11 antigen-like family member C, Leu M5, Leukocyte adhesion glycoprotein p150, 95 alpha chain, Leukocyte adhesion receptor p150, 95, CD antigen CD11c
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Konjugat: Allophycocyanin conjugate
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 3.9, monoclonal
• Speziesreaktivität: human
• Verpackung: pkg of 5 μL (0.25 μg/test)
• Methode(n): flow cytometry: suitable; immunofluorescence: suitable; immunoprecipitation (IP): suitable
• Isotyp: IgG1κ
• UniProt-Hinterlegungsnummer: P20702
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... ITGAX(3687)

Beschreibung

Allgemeine Beschreibung

The 3.9 antibody reacts with human CD11c, also known as integrin alpha X. This 150 kDa cell surface glycoprotein is part of a family of integrin receptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrins are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin receptors consist of distinct combinations of integrin chains which are differentially expressed. Integrin alpha X (CD11c) assembles with Integrin beta-2 (CD18) into a receptor known as CR4 which can bind and induce signaling through ICAMs and VCAM-1 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells. The 3.9 antibody is widely used as a marker for CD11c expression on dendritic cells (DC), often in parallel with markers for CD11b, for identification of developmental stages and mature subsets of this cell type. CD11c is prominently expressed on tissue macrophages, and is also detected on activated neutrophils, granulocytes, some types of activated T cells and intestinal intraepithelial lymphocytes (IEL). The antibody is reported to be cross-reactive with Baboon, Chimpanzee, Cynomolgus and Rhesus CD11c.

Immunogen

Immunogen information for this antibody is proprietary information.

Anwendung

Research Category
Entzündung & Immunologie

This mouse monoclonal antibody Anti-CD11c (human), APC & clone 3.9 validated for use in IP;Immunofluorescence;Flow Cytometry for the detection of CD11c.

Qualität

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 µg of this antibody detected CD11c in one million human peripheral blood monocytes.

Physikalische Form

Size Exclusion Chromatography

Purified mouse monoclonal antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.

Lagerung und Haltbarkeit

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable