Yes, low-fluorescent PVDF membrane is required in Licor IR Imaging Systems. The Immobilon-FL PVDF Membrane is suitable for this purpose. Immobilon-FL Membrane is the first transfer membrane optimized for fluorescence applications, with extremely low background that improves the sensitivity of all fluorescence detection protocols. It is compatible with all commonly used fluorescent probes at all excitation and emission wavelengths, making it ideal for multiplexing and chemifluorescence applications.
IPFL07810
Immobilon®-FL PVDF Membrane
1 roll, 7 x 8.4cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.
Synonym(e):
Western blotting membrane, blotting membrane, transfer membrane
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Über diesen Artikel
UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric
Pore size:
0.45 μm pore size
Compatibility:
for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red
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Unterstützung erhaltenProduktname
Immobilon®-FL PVDF-Transfermembran, 0.45 μm pore size, 7 X 8.4 cm sheets
material
PVDF membrane, plain filter, white filter
feature
hydrophobic
manufacturer/tradename
Immobilon®
technique(s)
dot blot: suitable, western blot: suitable
filter L × W
7 cm × 8.4 cm
pore size
0.45 μm pore size
capacity
155 μg/cm2 adsorption capacity (insulin), 205 μg/cm2 adsorption capacity (BSA), 300 μg/cm2 adsorption capacity (goat IgG)
compatibility
for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red
detection method
chemiluminescent, colorimetric, fluorometric
shipped in
ambient
Quality Level
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1 of 4
Dieser Artikel | IPFL10100 | IPFL00010 | IPFL20200 |
|---|---|---|---|
| material PVDF membrane, white filter, plain filter | material PVDF membrane, plain filter, white filter | material PVDF membrane, plain filter, white filter | material PVDF membrane, plain filter, white filter |
| pore size 0.45 μm pore size | pore size 0.45 μm pore size | pore size 0.45 μm pore size | pore size 0.45 μm pore size |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 400 |
| compatibility for use with Amido black, for use with Coomassie brilliant blue, for use with CPTS, for use with Ponceau-S red | compatibility for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red | compatibility for use with All fluorescence dyes, for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby | compatibility for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red |
| detection method chemiluminescent, fluorometric, colorimetric | detection method chemiluminescent, colorimetric, fluorometric | detection method chemiluminescent, colorimetric, fluorometric | detection method chemiluminescent, colorimetric, fluorometric |
| manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® |
General description
Die Immobilon®-FL-Transfermembran ist eine mikroporöse Polyvinylidenfluorid(PVDF)-Membran für die Bindung von Proteinen, die aus einer Reihe von Gelmatrizen übertragen wurden. Die Immobilon®-FL Membran hat eine nominale Porengröße von 0,45 μm und wird für das Blotting von Proteinen mit einem Molekulargewicht von mehr als 20 Kilodalton (kDa) empfohlen. Die Membran weist über ein breites Spektrum von Anregungs-/Emissionswellenlängen eine sehr geringe Autofluoreszenz auf. Durch diese Eigenschaft eignet sie sich ideal für Blotting-Anwendungen mit fluoreszenzbasiertem Immunnachweis.
Filtercode: IPFL
Filtercode: IPFL
Features and Benefits
Immobilon®-FL Transfermembran:
- Für optimale Fluoreszenz-Immunodetektionsanwendungen konzipiert
- Extrem niedriger Hintergrund verbessert die Empfindlichkeit aller fluoreszenzbasierten Immundetektionen
- Kompatibel mit allen gängigen Fluoreszenzsonden in einem breiten Spektrum von Anregungs-/Emissionswellenlängen
- Für standardmäßige Chemilumineszenz- oder chromogene Detektion
Legal Information
Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Verwandter Inhalt
There’s so much room for experimental variability in traditional immunodetection workflows. For your peace of mind – and ours – we designed the SNAP i.d.® 2.0 system to streamline your Western blot and immunohistochemistry experiments. The concept is simple: a vacuum-driven flow of blocking, antibody, and washing solutions reduces slide and membrane handling. That means a lot less shaking, dipping, pouring and waiting. And now you can process multiple blots and slides in parallel, so it’s easy to apply consistent conditions across experiments.
Protein Blotting Survival Handbook: Tips and Tricks
Antibody reuse for Western blotting is a common practice for many researchers. While many antibodies lose potency with time or degrade even faster due to improper storage conditions, it is important to recognize the potential value of recovering the primary antibody for possible reuse in some experiments. The SNAP i.d.® 2.0 system is not only able to reduce the immunodetection processing time, but its flexibility lets you combine conditions used in the standard immunodetection protocol and also allows the collection of antibody for future reuse. Here, we compare the antibody recovery and reuse in the standard immunodetection protocol with the antibody recovery and reuse in SNAP i.d.® system using the extended protocol and the original SNAP i.d.® protocol.
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Could you confirm if the IPFL07810 Immobilon membrane is compatible with a specific imaging system? I need to verify if this product will work for my requirements.
1 answer-
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