The thickness range of IPFL00010 is 110-145 microns.
IPFL00010
Immobilon® -FL PVDF Membrane
1 roll, 27 cm x 3.75 m, 0.45 µm pore size, transfer membrane with low background fluorescence
Immobilon®-FL PVDF Membrane
Synonym(e):
Western blotting membrane, blotting membrane, transfer membrane
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Über diesen Artikel
UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric
Pore size:
0.45 μm pore size
Compatibility:
for use with All fluorescence dyes, for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
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Unterstützung erhaltenProduktname
Immobilon®-FL PVDF Membran, 1 roll, 27 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.
material
PVDF membrane, plain filter, white filter
feature
hydrophobic
manufacturer/tradename
Immobilon®
technique(s)
dot blot: suitable, western blot: suitable
filter L × W
27 cm × 3.75 m
pore size
0.45 μm pore size
capacity
155 μg/cm2 adsorption capacity (insulin), 205 μg/cm2 adsorption capacity (BSA), 300 μg/cm2 adsorption capacity (goat IgG)
compatibility
for use with All fluorescence dyes, for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
detection method
chemiluminescent, colorimetric, fluorometric
shipped in
ambient
Quality Level
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1 of 4
Dieser Artikel | IPFL20200 | IPFL10100 | IPFL00005 |
|---|---|---|---|
| pore size 0.45 μm pore size | pore size 0.45 μm pore size | pore size 0.45 μm pore size | pore size 0.45 μm pore size |
| material PVDF membrane, white filter, plain filter | material PVDF membrane, plain filter, white filter | material PVDF membrane, plain filter, white filter | material PVDF membrane, plain filter, white filter |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 400 |
| detection method chemiluminescent, fluorometric, colorimetric | detection method chemiluminescent, colorimetric, fluorometric | detection method chemiluminescent, colorimetric, fluorometric | detection method chemiluminescent, colorimetric, fluorometric |
| compatibility for use with All fluorescence dyes, for use with CPTS, for use with Ponceau-S red, for use with Amido black, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Coomassie brilliant blue | compatibility for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red | compatibility for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red | compatibility for use with All fluorescence dyes, for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby |
| manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® |
General description
Die Immobilon®-FL-Transfermembran ist eine mikroporöse Polyvinylidenfluorid(PVDF)-Membran mit einer nominalen Porengröße von 0,45 μm. Sie wurde für die Bindung von Proteinen konzipiert, die aus einer Reihe von Gelmatrizen übertragen wurden. Die Immobilon®-FL-Membran ist hydrophob und bietet eine gleichmäßig kontrollierte Porenstruktur mit einer hohen Bindungskapazität für Biomoleküle. Die Membran ermöglicht das optimale Blotting von Proteinen mit Molekülmassen von mehr als 20.000. Die Immobilon®-FL-Membran weist über ein breites Spektrum von Anregungs-/Emissionswellenlängen eine sehr geringe Autofluoreszenz auf. Durch diese Eigenschaft eignet sie sich ideal für Blotting-Anwendungen mit fluoreszenzbasiertem Immunnachweis. Die Immobilon®-FL-Membran kann auch für den gängigen chemilumineszenten oder chromogenen Nachweis eingesetzt werden.
Features and Benefits
Immobilon-FL-Membran:
- Die erste Transfermembran, die für Fluoreszenzanwendungen optimiert wurde; der besonders geringe Hintergrund verbessert die Sensitivität aller Fluoreszenznachweisprotokolle
- Mit allen üblicherweise eingesetzten Fluoreszenzsonden mit einem breiten Spektrum von Anregungs- und Emissionswellenlängen kompatibel
- Am besten für das Multiplexen und den Chemifluoreszenznachweis
- Mit gängigen Blockierungsmitteln und -puffern kompatibel
Legal Information
Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Protokolle
Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.
Protokoll für die Probenvorbereitung zur Zelllyse und effizienten Proteinextraktion aus kultivierten Geweben und Zellen für das anschließende Western Blotting.
This page shows and discusses three protocols for stripping and reprobing a western blot membrane.
Verwandter Inhalt
Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.
There’s so much room for experimental variability in traditional immunodetection workflows. For your peace of mind – and ours – we designed the SNAP i.d.® 2.0 system to streamline your Western blot and immunohistochemistry experiments. The concept is simple: a vacuum-driven flow of blocking, antibody, and washing solutions reduces slide and membrane handling. That means a lot less shaking, dipping, pouring and waiting. And now you can process multiple blots and slides in parallel, so it’s easy to apply consistent conditions across experiments.
Antibody reuse for Western blotting is a common practice for many researchers. While many antibodies lose potency with time or degrade even faster due to improper storage conditions, it is important to recognize the potential value of recovering the primary antibody for possible reuse in some experiments. The SNAP i.d.® 2.0 system is not only able to reduce the immunodetection processing time, but its flexibility lets you combine conditions used in the standard immunodetection protocol and also allows the collection of antibody for future reuse. Here, we compare the antibody recovery and reuse in the standard immunodetection protocol with the antibody recovery and reuse in SNAP i.d.® system using the extended protocol and the original SNAP i.d.® protocol.
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What is the thickness of this product?
1 answer-
Helpful?
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Is it possible to use Immobilon FL instead of Immobilon P for western blots?
1 answer-
Yes, Immobilon FL can be used as an alternative to Immobilon P for western blots.
Helpful?
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Is 0.22 µm PVDF membrane available for fluorescent detection in Western blotting?
1 answer-
Immobilon PSQ, with a pore size of 0.2 µm, is suitable for various detection methods including fluorescent, radioactive, chromogenic, and chemifluorescent detection. But when using Immobilon PSQ in Western Blotting, it is important to consider that there may be increased background noise, which might require more stringent wash steps to ensure accurate results.
Helpful?
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Active Filters
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Great for western blots
I have been using this product for close to two and half years now. It is a highly reliable PVDF roll for western blotting. I use it to transfer a wide variety of proteins and use HRP conjugated secondary antibodies for imaging bands. The results have always been sharp, reliable and most importantly, reproducible. I shall buy again when we run out.
- A donation to charity was made for this review
- No
Recommends this productYesHelpful?
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