The kit has been verified for use with CSF and cell/tissue culture supernatants. Protein normalization is not required for these sample types. If using the kit with cell or tissue lysates, the sample will need to be normalized to total protein, using either a BCA or Coomassie-based assay. The product has not been validated for use with cell/tissue lysates.
HNS1MAG-95K
MILLIPLEX® Human Neuroscience Magnetic Bead Panel 1 - Neuroscience Multiplex Assay
allows quantitative multiplex detection of multiple analytes simultaneously
About This Item
Empfohlene Produkte
Qualitätsniveau
Speziesreaktivität
human
Hersteller/Markenname
Milliplex®
assay range
accuracy: 107%
(DJ1)
accuracy: 108%
(a-Synuclein)
accuracy: 123%
(TG2)
standard curve range: 12-50,000 pg/mL
(GFAP)
standard curve range: 15-60,000 pg/mL
(NSE)
standard curve range: 20-80,000 pg/mL
(UCHL1)
standard curve range: 244-1,000,000 pg/mL
(alpha-synuclein)
standard curve range: 61-250,000 pg/mL
(DJ1)
standard curve range: 61-250,000 pg/mL
(TG2)
Methode(n)
multiplexing: suitable
Nachweisverfahren
fluorometric (Luminex xMAP)
Versandbedingung
ambient
Allgemeine Beschreibung
MILLIPLEX® Human Neuroscience Bead Panel 1 is a 6-plex kit to be used for the simultaneous quantification of any or all of the following analytes in cerebrospinal fluid (CSF) samples: α-Synuclein, DJ1/PARK7, Glial fibrillary acidic protein (GFAP), Neuron specific enolase (NSE), Transglutaminase 2 (TGM2), and Ubiquitin carboxyl-terminal esterase L1 (UCHL1/PARK5).
Panel Type: Neuroscience
Spezifität
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Anwendung
- Analytes: GFAP, NSE, α-Synuclein, PARK5, Transglutaminase 2 (TGM2)
- Recommended Sample type: CSF
- Recommended Sample dilution: Neat
- Assay Run Time: Overnight or one day
- Research Category: Neuroscience
Leistungsmerkmale und Vorteile
Verpackung
Lagerung und Haltbarkeit
Sonstige Hinweise
Rechtliche Hinweise
Haftungsausschluss
Analysenzertifikate (COA)
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Verwandter Inhalt
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How can I normalize protein for this test? Just using same volume and total concentration protein are not reliable.
1 answer-
Helpful?
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Can serum or plasma samples be utilized in the Human Neuroscience Panel 1 kit, considering that the protocol exclusively mentions CSF?
1 answer-
The kit has not been validated with serum/plasma samples. Eight normal plasma samples were tested at a 1:4 dilution. It was found that GFAP and UCHL1 will require more sensitivity.
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