GF323

IGF-II, Human Recombinant
Animal Free

• Synonym(e): Insulin-like Growth Factor-II
• UNSPSC-Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.77

Eigenschaften

• Verunreinigungen: <0.1 μg/mg protein endotoxin (<1 EU/μg)
• Qualitätsniveau: 100
• UniProt-Hinterlegungsnummer: P01344
• Versandbedingung: dry ice

Beschreibung

Allgemeine Beschreibung

Product Source: Protein is expressed in E.coli.
The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.

The IGFs are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but, IGF-II can also signal through the IGFII/ Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions.
Recombinant human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and 3 intra-molecular disulfide bonds.

Anwendung

Research Category
Stammzellforschung

Research Sub Category
Wachstumsfaktoren & -rezeptoren

Qualität

Determined by its ability to stimulate the proliferation of mouse FDC-P1 cells. The expected ED50 is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥5 x 10^5 units/mg.

Physikalische Form

Product is sterile filtered through a 0.2 micron filter. Lyophilized with no additives.

Lagerung und Haltbarkeit

Store at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening.
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable