I3769

Insulin-like Growth Factor-I human

≥95% (HPLC), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(e): IGF, IGF-I, somatomedin c
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00081716
• Biological source: human
• Recombinant: expressed in E. coli
• Assay: ≥95% (HPLC)
• Form: lyophilized powder
• Mol wt: ~7.6 kDa
• Impurities: ≤1.00 EU/μg

Eigenschaften

• Produktname: Insulin-like Growth Factor-I human, IGF-I, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• biological source: human
• Quality Segment: 200
• recombinant: expressed in E. coli
• assay: ≥95% (HPLC)
• form: lyophilized powder
• potency: 0.1-10 ng/mL ED₅₀/EC₅₀
• quality: endotoxin tested
• mol wt: ~7.6 kDa
• packaging: pkg of 50 and 100 μg
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1.00 EU/μg
• UniProt accession no.: P05019
• storage temp.: −20°C
• Gene Information: human ... IGF1(3479), Igf1(16000)

Beschreibung

General description

Insulin-like growth factor I (IGF-I) is localized on human chromosome 12q23.2.[1]

Application

Insulin-like Growth Factor-I human has been used:

• in modified Dulbecco minimal essential medium for in vitro culture of testicular cells
• in culture media, to study its effect on steroidogenesis during testicular development
• in culture media for in vitro assay of splenic pro-erythroid cell growth and differentiation

Biochem/physiol Actions

IGF-I also known as somatomedin C, is secreted from the liver into circulation in a process regulated by pituitary growth hormone (GH) and so it mediates the growth-promoting activity of GH. In the developing embryo IGF-I is expressed primarily by mesenchymal-derived cells. After birth IGF-I expression in most extrahepatic tissues declines and hepatic expression of IGF-I becomes GH-regulated. Expression of IGF-I outside the liver is regulated differently, depending on the specific tissues. For example, gonadotropins and sex steroids regulate IGF-I expression within the reproductive system, while parathyroid hormone and sex steroids regulate IGF-I expression in bone. IGF-I is produced in several human tumors. IGF-I is mitogenic for a variety of cells including fibroblasts, osteoblasts, smooth muscle cells, fetal brain cells, neuroglial cells, and erythroid progenitor cells. IGF-I exerts its actions exclusively through the IGF-I receptor (IGF-IR). IGF-I induces endothelial cell migration and is involved in the regulation of angiogenesis.

IGF-I, also known as somatomedin C, mediates the growth-promoting activity of GH (growth hormone). IGF-I induces endothelial cell migration and is involved in the regulation of angiogenesis. IGF-I exerts its actions through the IGF-I receptor.

Insulin-like growth factor I (IGF-I) plays a critical role in cellular energy metabolism and growth and development, especially prenatal growth.[2]

Physical form

Lyophilized from 0.2 μm filtered solution in 50 mM acetic acid.

Preparation Note

Reconstitute in water

Analysis Note

The biological activiy is calculated by the dose-dependent proliferation of murine BALBC/3T3 cells. ED₅₀ corresponds to a specific activity of at least 1 x 10⁵ units/mg.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)