Our laboratory has not specifically studied the impact of high salt, such as 500 mM NaCl, on the activity of RNAseA. However, Park and Raines (2003) have investigated the activity of RNAseA under high salt concentration, up to 1 M NaCl. The results available in the publication titled "Catalysis by ribonuclease A is limited by the rate of substrate association" may serve as a useful reference. The study is available in Biochemistry, 2003 Apr 1;42(12):3509-18. From the study, it is noted that "the pKa values at 1.0 M NaCl are expected to be higher by 0.4-0.5 unit than the pKa values determined at 0.142 M NaCl."
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Quality Segment
description
Merck USA index - 14, 8202
assay
≥95% (SDS-PAGE)
form
lyophilized solid
specific activity
81-99 units/mg material
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated
solubility
water: 2 mg/mL
foreign activity
Protease, none detected
shipped in
ambient
storage temp.
−20°C
SMILES string
[nH]1cnc(c1)CC(NC(=O)CCN)C(=O)O
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI key
CQOVPNPJLQNMDC-UHFFFAOYSA-N
General description
chromatographisch aufgereinigte pyrimidinspezifische
Endoribonuklease,die auf einzelsträngige
RNA wirkt.
Preparation Note
Analysis Note
Other Notes
Legal Information
Disclaimer
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Dieser Artikel | |||
|---|---|---|---|
| description For selective degradation of RNA | description Type II-A, ≥60% (SDS-PAGE), >= 60 Kunitz units/mg protein | description (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0) | description Type III-A, ≥85% RNase A basis (SDS-PAGE), 85-140 Kunitz units/mg protein |
| assay ≥95% (SDS-PAGE) | assay - | assay - | assay ≥85% RNase A basis (SDS-PAGE) |
| specific activity 81-99 units/mg material | specific activity >= 60 Kunitz units/mg protein | specific activity - | specific activity 85-140 Kunitz units/mg protein |
| form lyophilized solid | form solid | form (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0) | form lyophilized powder |
| storage condition desiccated, OK to freeze | storage condition - | storage condition - | storage condition - |
| solubility water: 2 mg/mL | solubility - | solubility - | solubility - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Signalwort
Danger
Gefahrencodes
Sicherheitshinweise
Hazard Classifications
Resp. Sens. 1
Lagerklasse
11 - Combustible Solids
Was ist los?
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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What is the effectiveness of RNAseA when used with a buffer containing 500mM NaCl?
1 answer-
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