238407

Creatine Phosphokinase-MM Isoenzyme, Human Skeletal Muscle

Creatine Phosphokinase-MM Isoenzyme, Human Skeletal Muscle, is a native creatine phosphokinase-MM f that catalyzes the conversion of creatine and ATP to phosphocreatine and ADP.

• Synonym(e): hCPK-MM
• EG-Nummer: 2.7.3.2 (BRENDA, IUBMB)
• NACRES: NA.54
• UNSPSC Code: 12352202
• Specific activity: ≥300 units/mL

Eigenschaften

• Quality Segment: 100
• form: liquid
• specific activity: ≥300 units/mL
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• shipped in: wet ice
• storage temp.: −20°C

Beschreibung

General description

Native creatine phosphokinase-MM from human skeletal muscle. Catalyzes the reversible conversion of creatine and ATP to phosphocreatine and ADP. Plasma levels of CPK-MM are a useful indicator of acute myocardial infarction.

Physical form

In 10 mM Sodium Chloride, 5 mM Succinate, 5 mM β-Mercaptoethanol, 1 mM EDTA, 50% Glycerol, pH 7.1 +/- 0.1.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Other Notes

Baral, R., et al. 1993. Intern. Med.33, 210.
Wu, A.H.B. 1992. Lab. Med.23, 303.
Bergmeyer, H.U. 1974. Methods Enzymatic Anal.2, 789.

One unit is defined as the amount of enzyme that will catalyze the formation of 1 µmol of ATP from ADP and creatine phosphate per min at 37°C, pH 7.4.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable