900892

L-Azidohomoalanine hydrochloride

• Synonym(e): AHA
• Empirische Formel (Hill-System): C₄H₈N₄O₂ · HCl
• CAS-Nummer: 942518-29-8
• Molekulargewicht: 180.59
• MDL number: MFCD13184931
• UNSPSC Code: 12352209
• NACRES: NA.21

Eigenschaften

• form: powder or crystals
• Quality Segment: 100
• reaction suitability: reaction type: click chemistry
• mp: 139 °C
• storage temp.: −20°C
• SMILES string: [Cl-].[N+H3][C@@H](CCN=[N+]=[N-])C(=O)O
• InChI: 1S/C4H8N4O2.ClH/c5-3(4(9)10)1-2-7-8-6;/h3H,1-2,5H2,(H,9,10);1H/t3-;/m0./s1
• InChI key: MHHYJRIDKLZZEO-DFWYDOINSA-N

Beschreibung

Application

L-Azidohomoalanine (AHA) is an amino acid analog of methionine that contains a very small modification, specifically an azido moiety. This compound can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click ” reaction between an azide and an alkyne or cyclooctyne. For example the azido-modified protein can be detected with either fluorescent alkyne or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS/MS and MALDI-MS analysis.

Sicherheitshinweise

• Piktogramme: GHS02
• Signalwort: Danger
• Gefahrencodes: H242
• Sicherheitshinweise: P210 - P234 - P235 - P240 - P370 + P378 - P403
• Hazard Classifications: Self-react. C
• Lagerklasse: 5.2 - Organic peroxides and self-reacting hazardous materials
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable