Erythrosin B solutions can be prepared in various solvents. The dye is commonly prepared in water, buffered salt solutions or in ethanol. The Biological Stain Commission dissolves the dye in clove oil for the testing that is performed for certification of biological dyes. The solvent chosen will depend upon the staining applications. Erythrosin B can be dissolved in water to ~11 percent and 5% in ethanol. Some application recommend mixing or vortexing the solutions before use
Fortfahren mit
200964
Erythrosin B
Dye content ≥95 %
Synonym(e):
C.I. 45430:2, Solvent Red 140
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
5 g | Warenkorb auf Verfügbarkeit prüfen | € 61,60 |
25 g | Warenkorb auf Verfügbarkeit prüfen | € 248,00 |
Über diesen Artikel
Empirische Formel (Hill-System):
C20H8I4O5
CAS-Nummer:
Molekulargewicht:
835.89
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
240-046-0
Colour Index Number:
45430:2
MDL number:
Technischer Dienst
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Unterstützung erhaltenform
powder
Quality Segment
composition
Dye content, ≥95%
technique(s)
microbe id | staining: suitable
color
orange to red-orange
mp
303 °C (dec.) (lit.)
solubility
1 M NH4OH: 1 mg/mL, 1 M NH4OH: soluble 1 mg/mL, orange to red
ε (extinction coefficient)
≥102000 at 526-534 nm at 0.005 g/L (95% Methanol 5% 0.1M Na2CO3), ≥13000 at 310-318 nm in 1 M NH4OH at 0.005 g/L (95% Methanol 5% 0.1M Na2CO3), ≥32000 at 258-266 nm at 0.005 g/L (95% Methanol 5% 0.1M Na2CO3)
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
Oc1c(I)cc2c(Oc3c(I)c(O)c(I)cc3C24OC(=O)c5ccccc45)c1I
InChI
1S/C20H8I4O5/c21-11-5-9-17(13(23)15(11)25)28-18-10(6-12(22)16(26)14(18)24)20(9)8-4-2-1-3-7(8)19(27)29-20/h1-6,25-26H
InChI key
OALHHIHQOFIMEF-UHFFFAOYSA-N
Application
Erythrosin B has been used as a dye in fluorescence lifetime imaging.
Biochem/physiol Actions
Erythrosin B, also called as eosin B, is a xanthene dye. It belongs to the family of fluorescein dyes. It plays a major role in inhibiting dopamine uptake and high affinity 3H-ouabain binding and ion transport in synaptosomes from rat caudate nucleus.
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Dieser Artikel | |||
|---|---|---|---|
| description Dye content ≥95 % | description certified by the BSC, Dye content ≥85 % | description analytical standard | description certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland |
| technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) HPLC: suitable, gas chromatography (GC): suitable | technique(s) - |
| color orange to red-orange | color dark red | color - | color - |
| form powder | form powder | form - | form - |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) cleaning products | application(s) - |
| solubility 1 M NH4OH: 1 mg/mL, 1 M NH4OH: soluble 1 mg/mL, orange to red | solubility H2O: 1 mg/mL, clear | solubility - | solubility - |
| storage temp. room temp | storage temp. room temp | storage temp. - | storage temp. 2-8°C |
Lagerklasse
11 - Combustible Solids
Was ist los?
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
PSA (Persönliche Schutzausrüstung)
Eyeshields, Gloves, type N95 (US)
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How to prepare for Erythrosin B (200964) 0,5%?
1 answer-
Helpful?
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Are there instructions for using Erythrosin B for fluorescent microscopy?
1 answer-
Erythrosin B and Eosin B are considered synonyms; however, there are differences in their CAS numbers and CI numbers. Although there are no specific instructions for the use of product 200964, instructions are available for another Erythrosin B. This product can be used in a light microscopy procedure called hematoxylin and eosin staining. Additionally, both Erythrosin B and Eosin B are commonly used in H&E staining procedures and may be suitable for fluorescence microscopy for viability staining. While there is no product available for a staining procedure for viability staining, multiple procedures are available on the internet that can be considered for use with product 200964.
Helpful?
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