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product name
NCI-H69/LX10, 96042331
biological source
human lung
growth mode
Aggregates in suspension
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human small cell lung cancer, drug-resistant
Cell Line Description
The drug-resistant cell line NCI-H69/LX10 has been derived from the parent line NCI-H69 (ECACC No 91091802). It is recommended to culture the cells without drug upon resuscitation; add doxorubicine once growth is fully established.
Application
Drug-resistance studies
Culture Medium
RPMI 1640 + 2mM Glutamine + 1.0 μg/ml doxorubicine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2, 37ºC; resuscitate in drug-free medium and add drug after growth has been established.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
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