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Millipore

Thioglycollate Broth (USP Alternative)

suitable for microbiology, NutriSelect® Plus

Synonym(s):

Alternative Thioglycollate Medium, Fluid Thioglycollate Medium, NIH Thioglycollate Broth, TG Broth

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About This Item

UNSPSC Code:
41171607
NACRES:
NA.74

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

L-cystine, 0.5 g/L
dextrose, 5.5 g/L
pancreatic digest of casein, 15.0 g/L
sodium chloride, 2.5 g/L
sodium thioglycolate, 0.5 g/L
yeast extract, 5.0 g/L

manufacturer/tradename

NutriSelect® Plus

final pH

7.1±0.2 (25 °C)

application(s)

food and beverages
sterility testing
microbiology

suitability

anaerobic bacteria
nonselective for Aspergillus spp.
nonselective for Bacillus spp.
nonselective for Bacteroides spp.
nonselective for Candida spp.
nonselective for Clostridium spp.
nonselective for Escherichia coli
nonselective for Pseudomonas spp.
nonselective for Staphylococcus spp.
nonselective for bacteria (General Media)
nonselective for coliforms

Application

A medium for sterility tests and the cultivation of microaerophilic and anaerobic organisms.

Preparation Note

Suspend 29 g in 1 litre of distilled water. Bring to the boil and dissolve the medium completely. Distribute into tubes or bottles and sterilize by autoclaving at 121°C for 15 minutes. Prepare freshly or boil and cool the medium just before use!

Other Notes

Sterility tests

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCJ9044
BCCH0271
BCCD9908
BCCD9845
BCCB8494

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United States Pharmacopeia, 851-851 (1970)
N.I.H. Memorandum (1955)
William G Robichaux et al.
The Journal of pharmacology and experimental therapeutics, 360(3), 424-433 (2017-01-08)
Activator of G-protein signaling 4 (AGS4)/G-protein signaling modulator 3 (Gpsm3) contains three G-protein regulatory (GPR) motifs, each of which can bind G
Zhenghao Piao et al.
Inflammation, 40(3), 735-744 (2017-02-10)
Pseudomonas aeruginosa is the major cause of morbidity and mortality in patients with ventilator-associated pneumonia. Interferon regulatory factor 3 (IRF3) is a transcription factor that plays an important role in the immune response to viral infection via the IRF3/IFN-β signaling
Ah-Ra Jang et al.
Frontiers in immunology, 11, 1121-1121 (2020-06-26)
Helicobacter pylori is a gram-negative, microaerophilic, and spiral-shaped bacterium and causes gastrointestinal diseases in human. IL-1β is a representative cytokine produced in innate immune cells and is considered to be a key factor in the development of gastrointestinal malignancies. However

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