Functionalization of mesoporous silica nanoparticles with a cell-penetrating peptide to target mammalian sperm in vitro.

Functionalization of mesoporous silica nanoparticles with a cell-penetrating peptide to target mammalian sperm in vitro.

Nanomedicine (London, England) (2015-05-27)

Natalia Barkalina, Celine Jones, Helen Townley, Kevin Coward

RESUMEN

This study aimed to investigate the effects of actively targeting mesoporous silica nanoparticles (MSNPs) toward mammalian sperm with a cell-penetrating peptide (C105Y), with subsequent analysis of binding rates and nano-safety profiles. Boar sperm were exposed in vitro to C105Y-functionalized MSNPs or free C105Y, in a series of increasing doses for up to 2 h, followed by the evaluation of sperm motility, kinematic parameters, acrosome morphology, MSNP-sperm binding and cell fluorescence levels. C105Y-functionalized MSNPs preserved their biocompatibility with sperm, and exhibited an approximately fourfold increase in affinity toward gametes, compared with unmodified MSNPs, during the early stages of incubation. Our findings support the application of MSNPs and active targeting to sperm as valuable tools for reproductive biology.

MATERIALES

Referencia del producto

Marca

Descripción del producto

440140

Sigma-Aldrich

(3-aminopropil)trietoxisilano, 99%

H5882

Sigma-Aldrich

Bromuro de hexadeciltrimetilamonio, ≥98%

F8775

Sigma-Aldrich

Formaldehído solution, for molecular biology, 36.5-38% in H₂O

72068

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Hidróxido de sodio solution, BioUltra, for molecular biology, 10 M in H₂O

H9892

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Ácido clorhídrico solution, 1.0 N, BioReagent, suitable for cell culture

S2770

Sigma-Aldrich

Hidróxido de sodio solution, 1.0 N, BioReagent, suitable for cell culture

H6269

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Bromuro de hexadeciltrimetilamonio, for molecular biology, ≥99%

H1758

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Ácido clorhídrico, 36.5-38.0%, BioReagent, for molecular biology

2104

Supelco

Ácido clorhídrico solution, volumetric, 0.1 M HCl (0.1N), endotoxin free

H9151

Sigma-Aldrich

Bromuro de hexadeciltrimetilamonio, BioXtra, ≥99%

47608

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Formaldehído solution, for molecular biology, BioReagent, ≥36.0% in H₂O (T)

45726

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Ácido acético, for luminescence, BioUltra, ≥99.5% (GC)

741442

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(3-aminopropil)trietoxisilano, ≥98.0%

52365

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Bromuro de hexadeciltrimetilamonio, BioUltra, for molecular biology, ≥99.0% (AT)

W200611

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Ácido acético, ≥99.5%, FCC, FG

84429

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Ácido clorhídrico solution, ~6 M in H₂O, for amino acid analysis

71687

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Hidróxido de sodio, BioUltra, for luminescence, ≥98.0% (T), pellets

W200603

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Ácido acético, natural, ≥99.5%, FG

719803

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Carbon nanofibers, graphitized, platelets(conical), >98% carbon basis, D × L 100 nm × 20-200 μm

252549

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Formaldehído solution, ACS reagent, 37 wt. % in H₂O, contains 10-15% Methanol as stabilizer (to prevent polymerization)

705551

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Cloruro de hidrógeno solution, 3 M in cyclopentyl methyl ether (CPME)

322415

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Metanol, anhydrous, 99.8%

719811

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Carbon nanofibers, pyrolitically stripped, platelets(conical), >98% carbon basis, D × L 100 nm × 20-200 μm

W530574

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Ácido clorhídrico solution, 32 wt. % in H₂O, FCC

699632

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Carbon, mesoporous, nanopowder, less than 500 ppm Al, Ti, Fe, Ni, Cu, and Zn combined

151173

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Ninhydrin, ACS reagent

699624

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Graphite, nanopowder, graphitized, less than 250 ppm Al, Ti, Fe, Ni, Cu, and Zn combined