P6278
Peroxidase Inactivated
≤0.02 units/mg protein (biuret)
Sinónimos:
Peroxidase, inactivated from horseradish peroxidase
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10 MG
US$ 557,00
100 MG
US$ 2.650,00
US$ 557,00
Fecha estimada de envío29 de mayo de 2025
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10 MG
US$ 557,00
100 MG
US$ 2.650,00
About This Item
US$ 557,00
Fecha estimada de envío29 de mayo de 2025
Productos recomendados
Formulario
powder
Nivel de calidad
actividad específica
≤0.02 units/mg protein (biuret)
impurezas
salt, free
solubilidad
0.85% sodium chloride: soluble 15 mg/mL, clear to slightly hazy, colorless to light yellow
temp. de almacenamiento
2-8°C
Categorías relacionadas
Aplicación
Inactivated peroxidase is used to study regeneration of inactivated peroxidase enzymes[1]. It has been used to study the mechanism of peroxidase solubilization[2] and it is used to study antitumor activity of immobilized peroxidase systems[3]. It may be used for developing differential staining techniques in nerve tissue preparations.
Acciones bioquímicas o fisiológicas
HRP readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors. The optimal pH is 6.0-6.5 and the enzyme is most stable in the pH range of 5.0-9.0. HRP can be conjugated to antibodies by several different methods that include the use of glutaraldehyde, periodate oxidation, disulfide bonds, and also via amino- and thiol-directed cross-linkers. It is smaller and more stable than the enzyme labels, β-galactosidase and alkaline phosphatase. Hence, it is the most desired label. Also, its glycosylation leads to lower non-specific binding.[4] It is also used for the determination of glucose[5] and peroxides[6] in solution. Sodium azide, cyanide, L-cystine, dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and ions such as Cd2+, Co2+, Cu2+, Fe3+, Mn2+, Ni2+, and Pb2+ are found to inhibit the enzyme activity.[7]
Inactivated peroxidase reacts with goat and rabbit anti-peroxidase. It may be reactivated various methods such as heating[1].
Definición de unidad
One unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20°C.
Nota de preparación
Prepared from Type VI horseradish peroxidase by a modification of the method of Teale.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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C J Forehand et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 2(5), 654-652 (1982-05-01)
This study of spinal cord injury in bullfrog (Rana catesbeiana) tadpoles using the neuroanatomical tracer horseradish peroxidase (HRP) was undertaken to determine (1) whether the same anatomical regions that normally give rise to ascending or descending spinal tracts do so
S Morimoto et al.
The Journal of biological chemistry, 274(37), 26192-26198 (1999-09-03)
Elicitor treatment or mechanical damage to Scutellaria baicalensis Georgi (skullcap plants) callus causes an immediate insolubilization of a 36-kDa protein into cell walls. The 36-kDa protein was identified as peroxidase 1 by analysis of its internal amino acid sequence and
L L Vacca et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 23(3), 208-215 (1975-03-01)
Immunoperoxidase techniques are presented which can be used to localize horseradish peroxidase-tracer in paraffin-embedded tissues of the central nervous system. Compared to histochemical methods using frozen sections, these immunologic techniques allow the use of stored, serial paraffin sections, and appear
Dual projections of single neurons are visualized simultaneously: use of enzymatically inactive [3H]HRP.
N L Hayes et al.
Brain research, 165(2), 321-326 (1979-04-13)
Antitumor Activity of an Immobilized Peroxidase System Against Murine Ehrlich Ascites Is Mediated by the Immune System
Steven H. Combs, Clinton D. Morgan, et al.
Exp. Biol. Med, 204, 306-311 (1993)
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