Medio de Eagle modificado de Dulbecco, glucosa elevada, With 4500 mg/L glucose, stable glutamine, sodium pyruvate and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
esterilidad
sterile-filtered
Formulario
liquid
técnicas
cell culture | mammalian: suitable
impurezas
Endotoxin, tested
componentes
glucose: high HEPES: no stable glutamine sodium pyruvate: yes phenol red: yes NaHCO3: yes
This DMEM-Hi glucose medium differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.
Aplicación
El medio de Eagle modificado de Dulbecco (DMEM) es una modificación del medio basal Eagle (BME) que contiene el cuádruple de concentraciones de aminoácidos y vitaminas. La formulación original contenía 1000 mg/l de glucosa y se utilizaba para cultivar células embrionarias de ratón. Desde entonces, se ha modificado de varias formas para soportar cultivos primarios de células de ratón y de pollo, así como una variedad de células normales y transformadas. Cada uno de estos medios ofrece una combinación diferente de L-glutamina y piruvato sódico. Además, los niveles de glucosa se han elevado a 4 500 mg/l, lo que contribuye al nombre «DMEM/High».
Información legal
StableCell is a trademark of Sigma-Aldrich Co. LLC
American journal of physiology. Heart and circulatory physiology, 320(3), H1156-H1169 (2021-01-16)
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