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A4487

Aphidicolin

Ready Made Solution, from Nigrospora sphaerica

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Sinónimos:

ICI 69653, NSC-234714

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Talla/SKUDisponibilidadPrecio
1 mL
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US$ 253,00

Acerca de este artículo

Fórmula empírica (notación de Hill):
C20H34O4
Número CAS:
Peso molecular:
338.48
UNSPSC Code:
51102829
NACRES:
NA.85
EC Number:
200-664-3
MDL number:

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biological source

Nigrospora sphaerica

Quality Segment

vapor pressure

0.55 hPa ( 20 °C)

form

liquid, ready-to-use solution

color

clear colorless

solubility

H2O: miscible (completely)

antibiotic activity spectrum

neoplastics, viruses

mode of action

DNA synthesis | interferes, enzyme | inhibits

storage temp.

−20°C

SMILES string

O[C@]1([C@H]2C[C@@]3([C@@]4([C@H]([C@@]([C@@H](CC4)O)(CO)C)CC[C@H]3C2)C)CC1)CO

InChI

1S/C20H34O4/c1-17(11-21)15-4-3-13-9-14-10-19(13,7-8-20(14,24)12-22)18(15,2)6-5-16(17)23/h13-16,21-24H,3-12H2,1-2H3/t13-,14+,15-,16+,17-,18-,19-,20-/m0/s1

InChI key

NOFOAYPPHIUXJR-APNQCZIXSA-N

General description

Aphidicolin is a tetracyclic diterpene with antiviral and antimitotic properties used for cell cycle synchronization in various cell lines.

Application

Aphidicolin, from Nigrospora sphaerica, was used for cell cycle synchronization of tobacco BY-2 cells. It is used to block the entry of cells into S-phase.

Biochem/physiol Actions

Aphidicolin specifically inhibits DNA polymerase α that is responsible for DNA replication. It also inhibits α-like DNA polymerases of plants and yeasts but does not inhibit synthesis of RNA and proteins. Aphidicolin competes for the dCTP-specific binding site on DNA polymerase α specifically. Aphidicolin has antiviral and antimitotic properties. It blocks the cell cycle at S phase. It has been shown to induce apoptosis in HeLa cells.

Packaging

1 ml

Physical form

Aphidicolin Ready Made Solution supplied as a 1 mg/ml solution in DMSO.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Storage class (TRGS 510): Combustible liquids

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Este artículo
178273A0781SML3001
description

Ready Made Solution, from Nigrospora sphaerica

description

Aphidicolin, CAS 38966-21-1, is a cell-permeable antibiotic that acts as a cell synchronization agent. Blocks the cell cycle at early S-phase.

description

≥98% (HPLC), powder

description

0.2 μm filtered, 20mg/mL in 20mM HEPES

mode of action

DNA synthesis | interferes, enzyme | inhibits

mode of action

-

mode of action

DNA synthesis | interferes

mode of action

-

antibiotic activity spectrum

neoplastics, viruses

antibiotic activity spectrum

-

antibiotic activity spectrum

viruses, neoplastics

antibiotic activity spectrum

-

Quality Level

200

Quality Level

100

Quality Level

300

Quality Level

100

form

ready-to-use solution, liquid

form

solid

form

powder

form

liquid

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

solubility

H2O: miscible (completely)

solubility

ethanol: soluble, methanol: soluble, DMSO: 50 mg/mL

solubility

H2O: insoluble, ethanol: 1 mg/mL (stable at least a week at 4°C.), DMSO: 10 mg/mL (stable at least six weeks at −20°C.), methanol: 10 mg/mL

solubility

-


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Clase de almacenamiento

10 - Combustible liquids

Punto de inflamación (°F)

188.6 °F - closed cup

Punto de inflamación (°C)

87 °C - closed cup



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