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66807

Sigma-Aldrich

4,5-Methylenedioxy-1,2-phenylenediamine dihydrochloride

suitable for fluorescence, BioReagent, ≥97.0% (HPLC)

Sinónimos:

1,2-Diamino-4,5-methylenedioxybenzene dihydrochloride, 1,3-Benzodioxole-5,6-diamine dihydrochloride, 5,6-Diamino-1,3-benzodioxole dihydrochloride, MDB

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About This Item

Fórmula empírica (notación de Hill):
C7H8N2O2 · 2HCl
Número de CAS:
81864-15-5
Peso molecular:
225.07
Beilstein/REAXYS Number:
5641607
MDL number:
MFCD00037497
UNSPSC Code:
12352111
PubChem Substance ID:
329760322
NACRES:
NA.32

product line

BioReagent

assay

≥97.0% (HPLC)

form

solid

fluorescence

λex 367 nm; λem 445 nm in 0.1 M phosphate pH 7.0 (after derivatization with pyruvate)

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

Cl[H].Cl[H].Nc1cc2OCOc2cc1N

InChI

1S/C7H8N2O2.2ClH/c8-4-1-6-7(2-5(4)9)11-3-10-6;;/h1-2H,3,8-9H2;2*1H

InChI key

YXEJRYIEFFUUID-UHFFFAOYSA-N

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General description

4,5-Methylenedioxy-1,2-phenylenediamine dihydrochloride is a fluorescence tag for ⍺-keto acids. It is also required for the derivatization of sialic acids in presence of dilute sulfuric acid. The method allows determination of sialic acids.

Application

Fluorogenic reagent for α-ketoacids.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Caution

may discolor on storage

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
BCCL9233
BCCK5882
BCCH3237
BCCJ4199
BCCF6702

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S Hara et al.
Analytical biochemistry, 164(1), 138-145 (1987-07-01)
A simple, rapid, and highly sensitive high-performance liquid chromatographic method is described for the determination of N-acetyl- and N-glycolylneuraminic acids in human and animal sera, glycoproteins, and glycolipids. The neuraminic acids, released by acid hydrolysis of these biological samples, are
Capillary Electrophoresis of Carbohydrates: From Monosaccharides to Complex Polysaccharides, 249-249 (2010)
Xiaoxiao Guo et al.
The Analyst, 145(13), 4512-4521 (2020-05-16)
The polysialyltransferases (polySTs) catalyse the polymerisation of polysialic acid, which plays an important role in tumour metastasis. While assays are available to assess polyST enzyme activity, there is no methodology available specifically optimised for identification and quantitative evaluation of potential
K Hamahashi et al.
Journal of tissue engineering and regenerative medicine, 9(1), 24-30 (2012-11-21)
The authors aimed to repair and regenerate articular cartilage with layered chondrocyte sheets, produced using temperature-responsive culture dishes. The purpose of this study was to investigate the humoral factors produced by layered chondrocyte sheets. Articular chondrocytes and synovial cells were
Antonietta Manna et al.
Brain and behavior, 5(7), e00334-e00334 (2015-07-30)
Deep brain gray matter (GM) structures are involved in several neurodegenerative disorders and are affected by aging. In this study, we investigated the potential relationship between levels of brain-derived neurotrophic factor (BDNF), a putative biomarker of age- and clinically relevant
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