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15356

Sigma-Aldrich

Blotting-Nylon 66 membranes, type B, positive

for Northern and Southern blotting

Sinónimos:

Nylon 66-Blotting membranes, type B

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About This Item

UNSPSC Code:
41105339
NACRES:
NA.25

material

Nylon

grade

for molecular biology

form

roll

surface coverage

surface (quaternary ammonium groups) ≥99%

pore size

0.45 μm pore size

storage temp.

room temp

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General description

Positively charged nylon cast on polyester-support; membrane size 0,2m x 3m (rolled up)

Application

Suitable for:
Protein transfer
Radioactive detection of blotted proteins and nucleic acids
Sensitive dot and slot blots of nucleic acids
for high-sensitive nucleic acid blotting, dot and lot blots. Particularly suitable for radioactive detection. Suitable for protein transfer and immunoblotting.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Gerardas Dambrauskas et al.
Journal of experimental botany, 54(383), 699-706 (2003-01-30)
Very little is known about the expression patterns in plants of genes that encode proteins involved in the initiation of DNA replication. Partial cDNA sequences that encode Cdc6 and Mcm3 in tobacco have been isolated. The sequences were used as
K Pfeifer et al.
Journal of cell science, 106 ( Pt 2), 545-553 (1993-10-01)
Ubiquitination of proteins is a critical step in the controlled degradation process of many polypeptides. Here we show that sponges, the simplest multicellular group of eukaryotic organisms, are also equipped with the ubiquitin pathway. The polyubiquitin cDNA was isolated and

Protocolos

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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