Saltar al contenido
Merck

Documentos clave

Ver Toda la documentación

Saltar a

D0428

1 kb DNA Ladder

for DNA electrophoresis

Sinónimos:

1kb gel ladder, 1kb gel marker, 1kb ladder for gel electrophoresis, DNA gel marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker

Iniciar sesión para ver los precios por organización y contrato.

Seleccione un Tamaño

Cambiar Vistas
A ustedes/SKUDisponibilidadPrecio

Acerca de este artículo

NACRES:
NA.25
UNSPSC Code:
41105335
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarle

Quality Segment

100, 200

form

liquid

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

General description

Sigma′s 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. In nucleic acid gel electrophoresis, five μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting. All DNA markers can be stained with ethidium bromide, SYBR™ Green, and Nancy-520.

Application

1 kb DNA Ladder has been used as a molecular marker to determine the molecular weight and size of double stranded DNA during gel electrophoresis.[1]

Other Notes

For optimal resolution, the recommended agarose gel concentration is 0.75%.
Sigma′s 1kb Ladder is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Legal Information

SYBR is a trademark of Thermo Fisher Scientific or its subsidiaries

Clase de almacenamiento

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documentos section.

Si necesita más asistencia, póngase en contacto con Atención al cliente

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Questions

1–2 of 2 Questions  

  1. Does this product have biological origin? Or is the DNA synthetically formulated?

    1 answer

    1. This product has historically been synthetically produced. However, it is best to refer to the lot-specific Certificate of Origin, as the method of manufacture and origin of future lots may be subject to change. Please navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a Certificate under the ‘Certificate of Origin’ tab or click on the link below to request a lot-specific Certificate of Origin:
      https://www.sigmaaldrich.com/product/sigma/d0428#product-documentation

      Helpful?

  2. how to use this product. in what ratio we have to add dye in this.

    1 answer

    1. This product has been tested successfully at 2 µL, 5µL, and 7 µL., however for general use, a starting volume of 5 µL is recommended. Dilute 5 µL volume of D0428 with 25 µL of gel loading buffer. Product G2526 is used for testing purposes. For best results, a 0.75% gel is recommended.
      See the link below to Gel Loading Buffer, G2526:
      https://www.sigmaaldrich.com/product/sigma/g2526

      Helpful?

Reviews

No rating value

Active Filters