POLYDIC-RO

Poly[d(I-C)]

lyophilized, pkg of 10 U (10108812001 [A₂₆₀ units]), pkg of 50 U (11219847001 [A₂₆₀ units])

• Sinónimos: Poly-deoxy-inosinic-deoxy-cytidylic acid
• Código UNSPSC: 12352200

Propiedades

• descripción: Poly-deoxy-inosinic-deoxy-cytidylic acid
• Nivel de calidad: 100
• Formulario: lyophilized
• mol peso: 28814 bp
• envase: pkg of 10 U (10108812001 [A₂₆₀ units]); pkg of 50 U (11219847001 [A₂₆₀ units])
• fabricante / nombre comercial: Roche
• color: white
• mp: 54 °C (NaCl, 0.1 M, pH 7.0)
• solubilidad: water: soluble
• cociente de absorbancia: A_{290/260 nm} 0.24-0.32; A_{280/260 nm} 0.61-0.67; A_{250/260 nm} 1.18-1.30
• temp. de almacenamiento: −20°C

Descripción

Aplicación

Poly[d(I-C)] is used as a template for RNA polymerases, and as a DNA model for biophysical investigations.
It is also used as a primer template with Kornberg DNA polymerase for the determination of small amounts of dITP, dGTP, and dCTP. Poly(dI-dC) has been used in electrophoretic mobility shift assays (EMSA).[1]

Características y beneficios

Contents
Lyophilizate, sodium salt, base ratio 1:1, alternating base sequence

Calidad

Typical analysis:
Absorbance determination A₂₅₀/A₂₆₀, A₂₈₀/A₂₆₀, A₂₉₀/A₂₆₀

Secuencia

Chain Length approx. 20,000 bp

In an aqueous solution the DNA itself is a salt (the acid groups of the phosphate back bone have a negative charge and are saturated by positive sodium ions) i.e. there is no additional salt in solution. The structural formula which shows the negative 0-groups in the phosphate backbone is available. The same number of positive sodium ions must be dissociated in solution as counter ions.

As the sodium ions are part of the product and saturate the phosphate groups, the lyophilisate is salt-free.

Definición de unidad

1 A₂₆₀ unit corresponds to 50 μg ds DNA.

Unit Conversion: 1 A₂₆₀ unit corresponds to 50 μg ds DNA.

Nota de preparación

Activator: Polymines (spermine, spermidine)
Storage conditions (working solution): Solution is stable to at least 12 months if stored at 2 to 8 °C, contact with DNase or bacteria is to avoid.

Otras notas

For life science research only. Not for use in diagnostic procedures.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable