ABF23

Anti-NLRP3 Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: NACHT, LRR and PYD domains-containing protein 3, Angiotensin/vasopressin receptor AII/AVP-like, Caterpiller protein 1.1, CLR1.1, Cold autoinflammatory syndrome 1 protein, Cryopyrin, PYRIN-containing APAF1-like protein 1, NLRP3
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• técnicas: immunohistochemistry: suitable (paraffin); western blot: suitable
• Nº de acceso NCBI: NP_004886
• Nº de acceso UniProt: Q96P20
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... NLRP3(114548)

Descripción

Descripción general

NLRPs (Nucleotide-binding oligomerization domain, Leucine rich Repeat and Pyrin domain containing Proteins) are members of NLR (Nod-like receptors) protein family. NRLP genes are known to play important roles in both the mammalian reproductive system and the innate immune system. NLRP3 may function as an inducer of apoptosis, activator of NF-kappa-B signaling and activates caspase-1 in response to triggers such as bacterial or viral infection. NLRP3 defects are linked to familial cold autoinflammatory syndrome type 1 (FCAS1), Muckle-Wells syndrome (MWS) and chronic infantile neurologic cutaneous and articular syndrome (CINCA). NLRP3 is highly expressed in blood leukocytes, polymorphonuclear cells, osteoblasts and chondrocytes.

Especificidad

Other homologies: Porcine (80% sequence homology).

This antibody recognizes the DAPIN domain of NLRP3.

Inmunógeno

Epitope: DAPIN domain

KLH-conjugated linear peptide corresponding to the DAPIN domain of human NLRP3.

Aplicación

Detect NLRP3 using this rabbit polyclonal antibody, Anti-NLRP3 Antibody validated for use in western blotting & IHC (Paraffin).

Immunohistochemistry Analysis: A 1:4,000 dilution from a representative lot detected NLRP3 in human leukemia bone marrow and human tonsil tissue lysate.

Research Category
Inflammation & Immunology

Research Sub Category
Immunological Signaling

Calidad

Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected NLRP3 in 10 µg of THP-1 cell lysate.

Descripción de destino

~112 kDa and ~75 kDa observed. Five isoforms are known to exist at 118 kDa, 112 kDa, 112 kDa, 106 kDa, and 84 kDa This protein has been observed at ~75 kDa in some cell lysates (Kummer, J. A., et al. (2007). J Histochem Cytochem. 55(5):443-452.). Uncharacterized bands may be observed in some cell lysates.

Forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
THP-1 cell lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable