CS0780
β-N-Acetylglucosaminidase Assay Kit
sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL)
Sinónimos:
Beta-NAG Activity Assay Kit
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Productos recomendados
usage
sufficient for 50 reactions (1 mL)
sufficient for 500 reactions (100 μL)
Quality Level
shipped in
wet ice
storage temp.
2-8°C
Gene Information
human ... NAGLU(4669)
Categorías relacionadas
Application
The kit provides the reagents required for a fast and convenient detection of β-N-Acetylglucosaminidase activity in cell lysates, tissue homogenates or purified enzyme. It is a useful tool for the detection of lysosomes in fractionated cell/tissue samples.
Biochem/physiol Actions
N-acetyl-D-glucosaminidase (NAG) in mammals is a lysosomal enzyme, which takes part in the intracellular degradation of glycolipids and glycoproteins. High activities of this enzyme have been detected in human kidney, lung and liver lysosomes. Elevated levels of serum NAG are associated with certain forms of cancer.
Analysis Note
The kit assay is based on the hydrolysis of NAG substrate (NP-GLcNAc) by the enzyme. The enzymatic hydrolysis of the substrate liberates p-nitrophenylate ion. The reaction product is detected colorimetrically at 405 nm.
Solo componentes del kit
Referencia del producto
Descripción
- Dilution Buffer 8 mL
- 4-Nitrophenyl-N-acetyl-β-D-glucosaminide 50 mg
- Citrate Buffer Solution, 0.09 M 100 mL
- p-Nitrophenol Standard Solution, 10 mM 1 mL
- β-N-Acetylglucosaminidase from Jack beans 1 vial
- Sodium carbonate 5 g
related product
Referencia del producto
Descripción
Precios
signalword
Warning
hcodes
Hazard Classifications
Carc. 2 - Eye Irrit. 2 - STOT RE 2 Oral
target_organs
Liver,Kidney
Storage Class
11 - Combustible Solids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Shanshan Wang et al.
Biochemical and biophysical research communications, 483(1), 45-51 (2017-01-09)
Activation of endothelial cells plays a key role in septic acute kidney injury (AKI). This study investigated the role of miRNA in endothelial-induced tubular cell injury in sepsis. Circulating endothelial cells (CECs) from septic AKI, non-septic AKI, septic non-AKI patients
Miguel A Lanaspa et al.
Journal of the American Society of Nephrology : JASN, 25(11), 2526-2538 (2014-05-31)
Diabetes is associated with activation of the polyol pathway, in which glucose is converted to sorbitol by aldose reductase. Previous studies focused on the role of sorbitol in mediating diabetic complications. However, in the proximal tubule, sorbitol can be converted
Na Zhang et al.
Cell death & disease, 9(2), 230-230 (2018-02-16)
Glycogen synthase kinase-3β (GSK-3β) is a ubiquitously expressed serine/threonine kinase involved in a variety of functions ranging from the control of glycogen metabolism to transcriptional regulation. We recently demonstrated that GSK-3β inhibition triggered ASK1-JNK-dependent apoptosis in human hepatocellular carcinoma (HCC)
Qiuyuan Yin et al.
The Journal of cell biology, 219(8) (2020-07-15)
Lysosomes are degradation and signaling organelles that adapt their biogenesis to meet many different cellular demands; however, it is unknown how lysosomes change their numbers for cell division. Here, we report that the cyclin-dependent kinases CDK4/6 regulate lysosome biogenesis during
Di Chen et al.
Developmental cell, 55(5), 588-602 (2020-12-09)
Liquid-liquid phase separation (LLPS) compartmentalizes transcriptional condensates for gene expression, but little is known about how this process is controlled. Here, we showed that depletion of IPMK, encoding inositol polyphosphate multikinase, promotes autophagy and lysosomal function and biogenesis in a
Artículos
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico