803561

BMOE (bis-maleimidoethane)

• Sinónimos: 1,1′-ethane-1,2-diylbis(1H-pyrrole-2,5-dione), N,N′-Ethylenebismaleimide, BMOE, Ethylenebismaleimide
• Fórmula empírica (notación de Hill): C₁₀H₈N₂O₄
• Número de CAS: 5132-30-9
• Peso molecular: 220.18
• MDL number: MFCD00458699
• UNSPSC Code: 12352200
• PubChem Substance ID: 329768818
• NACRES: NA.22

Propiedades

• form: powder
• mol wt: 220.18
• reaction suitability: reagent type: cross-linking reagent
• storage condition: desiccated
• solubility: DMSO or DMF: soluble
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: O=C(C=CC1=O)N1CCN2C(C=CC2=O)=O
• InChI: 1S/C10H8N2O4/c13-7-1-2-8(14)11(7)5-6-12-9(15)3-4-10(12)16/h1-4H,5-6H2
• InChI key: PUKLCKVOVCZYKF-UHFFFAOYSA-N

Descripción

General description

BMOE, BMB and BMH are homobifunctional, maleimide crosslinkers for conjugation between sulfhydryl groups (-SH). Such bismaleimide crosslinkers are commonly used to explore and characterize protein structure (i.e., oligomerization) or protein interactions. Because BMOE, BMB and BMH have the same reactivity but differ in length, the relative success of these three reagents in forming crosslinks between sites in a protein oligomer or interaction can assist in determining intra- and intermolecular distances.

Features and Benefits

• Reactive groups: maleimide (both ends)
• Reactive towards: sulfhydryl groups
• Short (8.0Å), sulfhydryl-to-sulfhydryl crosslinker, composed of maleimide groups and 7-atom spacer arm
• Shortest bismaleimide crosslinker available for close proximity crosslinking
• Water-insoluble—dissolve first in DMF or DMSO, then add to aqueous reaction buffers
• Noncleavable

Caution

This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable