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Merck

219215

Sigma-Aldrich

Leucocrystal Violet

Sinónimos:

4,4′,4′′-Methylidynetris(N,N-dimethylaniline), Leuco Crystal Violet

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5 G
US$ 107,00
25 G
US$ 276,00

US$ 107,00

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5 G
US$ 107,00
25 G
US$ 276,00

About This Item

Fórmula lineal:
CH[C6H4N(CH3)2]3
Número de CAS:
Peso molecular:
373.53
Número CE:
Número MDL:
Código UNSPSC:
12171500
ID de la sustancia en PubChem:
NACRES:
NA.47

US$ 107,00

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Formulario

powder

Nivel de calidad

técnicas

microbe id | staining: suitable

mp

175-177 °C (lit.)

λmáx.

260 nm

ε (coeficiente de extinción)

≥700 at 257-263 nm

aplicaciones

diagnostic assay manufacturing
hematology
histology

temp. de almacenamiento

room temp

cadena SMILES

CN(C)c1ccc(cc1)C(c2ccc(cc2)N(C)C)c3ccc(cc3)N(C)C

InChI

1S/C25H31N3/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6/h7-18,25H,1-6H3

Clave InChI

OAZWDJGLIYNYMU-UHFFFAOYSA-N

Aplicación

Leucocrystal Violet has been used to identify histamine-producing lactobacilli in cheese.[1]

Acciones bioquímicas o fisiológicas

Leucocrystal Violet (LCV) is a cationic triarylmethane dye. LCV is a reduced form of gentian violet (crystal violet) and thus, it is colorless. It has an affinity for both cellulosic and proteinaceous materials. LCV and hydrogen peroxide react with the hemoglobin in blood and turn the blood impression to purple/violet color. Therefore, the dye is widely used to stain blood residue on both porous and non-porous materials.[2][3]

Precaución

Photo-ionization can cause color variations

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Referencia del producto
Descripción
Precios

Pictogramas

Environment

Palabra de señalización

Warning

Frases de peligro

Consejos de prudencia

Clasificaciones de peligro

Aquatic Acute 1 - Aquatic Chronic 1

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Gloves


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J E Roybal et al.
Journal of chromatography, 467(1), 259-266 (1989-04-21)
High-performance liquid chromatographic conditions are reported for the electrochemical detection (ED) of Gentian Violet, its demethylated metabolites, Leucogentian Violet and Methylene Blue. Gentian Violet, its demethylated metabolites and Leucogentian Violet were separated within 14 min on a cyano column eluted
Xueli Wu et al.
Journal of chromatography. A, 1172(2), 121-126 (2007-10-24)
A method has been developed for the simultaneous determination of malachite green, gentian violet and their leuco-metabolites in various aquatic products using isotope dilution liquid chromatography-linear ion trap mass spectrometry without post-column oxidation. Sample was extracted with McIlvaine buffer and
J E Roybal et al.
Journal - Association of Official Analytical Chemists, 73(6), 940-946 (1990-11-01)
A method is presented for determination of residues of gentian violet (GV), its demethylated metabolites (pentamethyl and tetramethyl), and leucogentian violet (LGV) in chicken tissue. The analytes are extracted from tissue with acetonitrile/buffer and partitioned into methylene chloride. Polar lipids
R T Wilson et al.
Journal of AOAC International, 77(5), 1137-1142 (1994-09-01)
A gas chromatographic/mass spectrometric (GC/MS) procedure for confirming the identity of leucogentian violet (LGV) in chicken fat was developed for regulatory application. The unused portion of the extract remaining from a determinative procedure was back-extracted into an organic phase, concentrated
J J McDonald et al.
Drug metabolism and disposition: the biological fate of chemicals, 12(3), 330-336 (1984-05-01)
Cellular suspensions of microflora isolated from human feces and from intestinal contents of rat and chicken transformed the triphenylmethane dye gentian violet under anaerobic conditions. Pure cultures of nine genera of strict and facultative anaerobes were also active. All pure

Questions

1–2 of 2 Questions  
  1. Is 219215-5G the same powder that is used in the urinalysis dip sticks?

    1 answer
    1. Leucocrystal violet is the completely reduced form of crystal violet. The dye powder appears white to gray to lavender, and solutions are expected to be colorless. In addition to identifying lactobacilli in cheese, Leucocrystal Violet (LCV) is a cationic triarylmethane dye that has an affinity for both cellulosic and proteinaceous materials. It reacts with the hemoglobin in blood and turns the blood impression to a purple/violet color. Consequently, the dye is widely used to stain blood residue on both porous and non-porous materials. There is also mention of Leucocrystal Violet being used as an indicator on a urinalysis test strip, potentially to detect the presence of blood or protein. However, the quality level for Leucocrystal Violet is 200 on a scale of 100 to 600, and it is only advertised as suitable for Research Use Only. Urinalysis test strips sold for human use are classified as medical devices and are stated to be suitable for In Vitro Diagnostic Use. As such, product 219215 is not suitability tested to ensure the dye is suitble for use in the manufacture of urinalysis test strips.

      Helpful?

  2. What is the purity of lot/batch #:MKBW1177V

    1 answer
    1. The purity is not determined for this product, only carbon and nitrogen are quantitated.

      Helpful?

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