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MAK118

Sigma-Aldrich

Invertase Activity Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

β-fructofuranosidase Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

application(s)

cosmetics
environmental
food and beverages

detection method

colorimetric
fluorometric

storage temp.

−20°C

General description

Invertase (β-fructofuranosidase) is an enzyme that catalyzes the hydrolysis of sucrose to fructose and glucose. Invertase is produced by certain plants, microorganisms, and honey bees.

Application

Invertase Assay Kit has been used for screening inhibition of recombinant invertase inhibitor protein from sugarcane.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the detection of invertase activity in biological and environmental samples and for the screening of invertase inhibitors.

Principle

In this kit, invertase activity is determined by a coupled enzyme assay in which invertase cleaves sucrose to glucose and fructose, resulting in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the invertase activity present. One unit of Invertase is the amount of enzyme that will catalyze the formation of 1.0 μmole of glucose per minute at pH 4.5 under the assay conditions.

Storage Class Code

10 - Combustible liquids


Certificates of Analysis (COA)

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Invertase can catalyze the hydrolysis of sucrose, and is widely distributed in cells of cyanobacteria and plants. Being responsible for the first step for sucrose metabolism, invertase plays important physiological roles and its enzymatic activity is frequently needed to be

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