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Enzymatic Activity Assay for Invertase in Synechocystis Cells.

Bio-protocol (2018-05-20)
Xiaoming Tan, Kuo Song, Xuefeng Lu
ABSTRACT

Invertase can catalyze the hydrolysis of sucrose, and is widely distributed in cells of cyanobacteria and plants. Being responsible for the first step for sucrose metabolism, invertase plays important physiological roles and its enzymatic activity is frequently needed to be determined. All the methods for determination of the invertase activity are dependent on detection of the glucose product generated by the invertase. Here we describe an ion chromatography based protocol of our laboratory for determination of cyanobacterial intracellular invertase activity.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Phenylmethanesulfonyl fluoride, ≥98.5% (GC)
Pricing and availability is not currently available.
Sigma-Aldrich
Invertase Activity Assay Kit, sufficient for 100 colorimetric or fluorometric tests
Pricing and availability is not currently available.