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M7633

Sigma-Aldrich

4-Methylumbelliferyl α-D-galactopyranoside

≥98% (TLC)

Synonym(s):

MU-alpha-GAL

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About This Item

Empirical Formula (Hill Notation):
C16H18O8
CAS Number:
38597-12-5
Molecular Weight:
338.31
Beilstein:
94674
EC Number:
254-031-1
MDL number:
MFCD00063278
UNSPSC Code:
12352204
PubChem Substance ID:
24897170
NACRES:
NA.32

Quality Level

200

Assay

≥98% (TLC)

form

powder

solubility

water: 50 mg/mL, clear, colorless to very faintly yellow

fluorescence

λex 317 nm; λem 374 nm (pH 9.1)
λex 360 nm; λem 449 nm (Reaction product)

storage temp.

−20°C

SMILES string

CC1=CC(=O)Oc2cc(O[C@H]3O[C@H](CO)[C@H](O)[C@H](O)[C@H]3O)ccc12

InChI

1S/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13+,14+,15-,16+/m1/s1

InChI key

YUDPTGPSBJVHCN-CHUNWDLHSA-N

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Application

4-Methylumbelliferyl α-D-galactopyranoside has been used as an artificial substrate to assay α-galactosidase-A activity.

Biochem/physiol Actions

4-Methylumbelliferyl α-D-galactopyranoside is a fluorogenic substrate for α-galactosidase-A. The enzyme converts the substrate to form a blue fluorescent product, methylumbelliferyl. The fluorescence is measured spectrophotometrically.

Preparation Note

4-Methylumbelliferyl α-D-galactopyranoside is soluble in water (50 mg/ml), yielding a clear, colorless to faint yellow solution with the application of heat.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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This study illustrates how optimization of both liquid-handling accuracy and precision is critical to assay performance. The study was designed to examine (1) liquid-handling performance and (2) the effect of liquid-handling variability on two types of in vitro biochemical assays
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