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5051

Sigma-Aldrich

Human Vitronectin

from human plasma, liquid, 0.5 mg/mL, suitable for cell culture

Synonym(s):

Cell adhesion protein, ECM protein

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.75

product name

Vitronectin, from human plasma, suitable for cell culture, solution

biological source

human plasma

Quality Level

100

sterility

sterile-filtered

Assay

>95%

form

solution

mol wt

75 kDa

packaging

pkg of 0.1 mg

concentration

0.5 mg/mL

technique(s)

cell culture | mammalian: suitable

solubility

water: miscible

UniProt accession no.

P04004

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... VTN(7448)

General description

Vitronectin is a 75 kDa glycoprotein produced by hepatocytes and liberated into the blood. It has four domains and at least two structural motifs. Vitronectin gene is small with three kilobases in length and has eight exons. It is also present on fibers in the extracellular matrices of several tissues.
Vitronectin is a monomeric glycoprotein used to promote cell attachment, migration, proliferation and differentiation in a broad number of cell lines and types. This product has been purified from human plasma where it is found as a mixture of 75kDa and 65kDa polypeptides.

Vitronectin′s primary use in cell culture is related to cell adhesion. It also binds to heparin and collagen.

Vitronectin is ideal for coating of surfaces. The optimal concentration for cell attachment and culture may differ for various cell types. Vitronectin has been used at a final coating concentration as low as 50 ng/cm2 on plasticware. It is provided in user-friendly packaging for use and storage. Vitronectin is sterile filtered and is supplied as a ready to use solution after thawing and concentration adjustment.

Biochem/physiol Actions

Vitronectin controls proteolysis. It helps in in vitro cell adhesion.Vitronectin is involved in the modulation of perforin mediated killing by cytotoxic T-lymphocytes and natural killer (NK) cells. It controls the inflammatory reactions in blood.

Preparation Note

This product is supplied in a solution of 0.15 M NaCl and 0.005M HEPES at pH 7.4

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

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Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

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