CC102A

Human Integrin αVβ3, Octyl-β-D-glucopyranoside Formulation

• Synonym(s): Vitronectin Receptor, CD51/CD61
• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.41

Properties

• biological source: human
• Quality Level: 100
• Assay: >95% (silver stain, SDS-PAGE)
• form: liquid
• manufacturer/tradename: Chemicon^®
• concentration: 0.25 mg/mL
• NCBI accession no.: NM_002210.2
• UniProt accession no.: P06756
• Gene Information: human ... ITGAV(3685)

Description

General description

Integrin alphaVbeta3 was purified from human placenta by affinity chromatography using immobilized monoclonal antibodies to alphaVbeta3 integrin. A tissue detergent extract, which was applied to the column was prepared as previously described [Belkin, 1990]. Product was tested and found negative for HIV and Hepatitis B.

Application

Electrophoresis and Immunoblotting control. Also useful for in vitro binding experiments with metalloproteinase MMP-2 .

Physical form

Purified protein inTris buffered saline with 100 mM Octyl-beta-D-glucopyranoside, and sodium azide. This format of alphaVbeta3 is appropriate for functional evaluations.

Storage and Stability

Maintain at -70°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/ thaw cycles.

Analysis Note

Specific Activity: Confirmed by vWf (von Willibrand factor) binding assay. It is expected that this preparation of alphaVbeta3 integrin will also interact with other alphaVbeta3 binding proteins in an RGD-dependent fashion.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable