Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

PE0230

Sigma-Aldrich

Plant Total Protein Extraction Kit

Suitable for any plant species or tissue

Synonym(s):

Total Protein Extraction Kit

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41105500
NACRES:
NA.56
Pricing and availability is not currently available.

Quality Level

200

technique(s)

protein extraction: suitable

storage temp.

−20°C

General description

The Plant Total Protein Extraction Kit is designed specifically for use in plant bioscience to extract a qualitative sample of all proteins from any type of plant species or tissue. The protocol does not require any ultracentrifugation or aqueous polymer two-phase partitioning (APTP). The kit includes two reagents; a plant specific protease inhibitor cocktail and a new chaotropic reagent with increased solubilizing power to extract more hydrophobic proteins (C0356, Protein Extraction Reagent Type 4). Routine use of the reagent in sequential protein extraction of a plant tissue sample with fresh reagent removes nearly all protein from the tissue. The protease inhibitor cocktail is a mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, metalloproteases, aspartic, and aminopeptidases. It contains pepstatin A, 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), E-64, leupeptin, 1,10-phenanthroline, and bestatin. The cocktail has been demonstrated to be highly effective in preventing protein degradation during the extraction process.

Following removal of polyphenolics, tannins, and other interfering substances, ground plant tissue, fresh or frozen, is resuspended in the chaotropic reagent. Plant debris is pelleted by centrifugation and the protein extract is collected. The end result is a qualitative total protein sample, ready for downstream proteomic analysis.

Application

The plant total Protein Extraction Kit has been used to isolate crude seed protein.[1] It has also been used to extract the total protein.[2][3]

Quantity

Sufficient for preparation of 20 separate extractions from plant tissue samples of 10-250mg

Kit Components Also Available Separately

Product No.
Description
SDS
  • C0356Protein Extraction Reagent Type 4 1 bottleSDS
  • P9599Protease Inhibitor Cocktail, for plant cell and tissue extracts, DMSO solution 1 mLSDS

Signal Word

Warning

Hazard Statements

H302,H351,H361fd,H411

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Repr. 2

Storage Class Code

11 - Combustible Solids

Flash Point(F)

185.0 °F - closed cup

Flash Point(C)

85 °C - closed cup

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000305032
0000305032
0000263820
0000263820
SLCP4303

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Samuel L Chen et al.
Frontiers in plant science, 10, 1116-1116 (2019-10-15)
Pre-mRNA alternative splicing is a conserved mechanism for eukaryotic cells to leverage existing genetic resources to create a diverse pool of protein products. It is regulated in coordination with other events in RNA metabolism such as transcription, polyadenylation, RNA transport
Xu Zeng et al.
Botanical studies, 59(1), 8-8 (2018-03-08)
Gastrodia elata, a mycoheterotrophic orchid, is a well-known medicinal herb. In nature, the seed germination of G. elata requires proper fungal association, because of the absence of endosperm. To germinate successfully, G. elata obtains nutrition from mycorrhizal fungi such as
Revealing proteins associated with symbiotic germination of Gastrodia elata by proteomic analysis
Zeng X, et al.
Botanical studies, 59(1), 8-8 (2018)
Bioactivity-Guided Isolation of Neuritogenic Factor from the Seeds of the Gac Plant (Momordica cochinchinensis)
Mazzio E, et al.
Evidence-Based Complementary and Alternative Medicine : ECAM, 2018 (2018)
Xing-Min Geng et al.
Frontiers in plant science, 7, 26-26 (2016-02-10)
Production of reactive oxygen species (ROS) can be accelerated under various biotic and abiotic stresses causing lipid peroxidation, protein degradation, enzyme inactivation, and DNA damage. Superoxide reductase (SOR) is a novel antioxidant enzyme from Pyrococcus furiosus and is employed by
View All Related Papers

Questions

1–4 of 4 Questions  

  1. How can I improve my yield when using Product PE0230, Plant Protein Extraction Kit?

    1 answer

    1. A second extraction can be performed and combined with the first to increase protein extracted to about 95% of the total.

      Helpful?

  2. What is the expected protein yield when using Product PE0230, Plant Protein Extraction Kit?

    1 answer

    1. Depending on the tissue used (age, type (leaf, stem, root) health, etc.) one can expect 1-4 mg/mL at about 75-80% of the total protein.

      Helpful?

  3. Will my protein samples be denatured when using Product PE0230, Plant Protein Extraction Kit?

    1 answer

    1. Yes. The chaotropic agents and the detergent in the kit will denature the proteins at least partially.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service