Yes, SYPRO Ruby Protein Blot Stain works with both nitrocellulose and polyvinylidene difluoride (PVDF) membranes. For more information, please access the Product Data Sheet which can be found in the DOCUMENTATION section under the 'More Documents' tab:
https://www.sigmaaldrich.com/product/sigma/s4942#product-documentation
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/369/103/s4817dat.pdf
S4942
SYPRO™ Ruby Protein Gel Stain
Synonym(s):
protein gel stain, SYPRO™ dye
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 200 mL | Available to ship TODAYfromMILWAUKEE | $191.00 | |
| 1 L | Available to ship TODAYfromMILWAUKEE | $651.00 | |
| 5 L | Available to ship TODAYfromMILWAUKEE | $2,640.00 |
About This Item
NACRES:
NA.32
UNSPSC Code:
41105322
$191.00
Available to ship TODAYDetails
Subscriptions are currently unavailable.
Ships Every 4 weeks
shelf life
≥6 mo. (when stored at room temperature and protected from light)
Quality Level
technique(s)
protein staining: suitable
fluorescence
λex 280,450 nm; λem 610 nm
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) protein staining: suitable | technique(s) protein staining: suitable | technique(s) protein staining: suitable | technique(s) PCR: suitable |
| fluorescence λex 280,450 nm; λem 610 nm | fluorescence λex 300,470 nm; λem 570 nm | fluorescence - | fluorescence - |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| shelf life ≥6 mo. (when stored at room temperature and protected from light) | shelf life ≥6 mo. (when stored desiccated and protected from light at room temperature, 4 °C or 20 °C.) | shelf life - | shelf life - |
General description
SYPRO Ruby protein gel stain is a ready-to-use, ultrasensitive, luminescent stain for the detection of proteins separated by polyacrylamide gel electrophoresis (PAGE). This stain, designed especially for use in 2-D PAGE, has proven to be an excellent choice for 1-D PAGE and isoelectric focusing (IEF) gels as well. SYPRO Ruby protein gel stain attains sensitivity comparable to many silver stain techniques. However, unlike silver staining, the SYPRO Ruby gel stain:
- uses a simple staining protocol, with no possibility of overstaining
- delivers a linear quantitation range of over three orders of magnitude
- shows less protein-to-protein variability
- stains glycoproteins, lipoproteins, calcium-binding proteins, fibrillar proteins, and other difficult-to-stain proteins
- will not stain extraneous nucleic acids
- does not interfere with subsequent analysis of proteins by Edman-based sequencing or mass spectrometry
Application
SYPRO ruby protein gel stain has been used for staining of the proteins after sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE).[1]
Legal Information
SYPRO is a trademark of Thermo-Fisher Scientific, Inc.
Disclaimer
Protect from light.
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Silicatein-mediated incorporation of titanium into spicules from the demosponge Suberites domuncula.
Filipe Natalio et al.
Cell and tissue research, 339(2), 429-436 (2009-12-17)
Primmorphs (a three-dimensional sponge primary cell culture system) have been revealed to be a cell/tissue nano-factory for the production of tailor-made hybrid nanostructures. Growth of primmorphs is stimulated by the presence of a titanium alkoxide precursor tolerating titania (TiO2) concentrations
Chronic hypoxia alters mitochondrial composition in human macrophages.
Fuhrmann DC, et al.
Biochimica et Biophysica Acta, 1834, 2750-2760 (2013)
Jun Zou et al.
eLife, 4, e09406-e09406 (2015-10-17)
Truncating mutations in the giant sarcomeric protein Titin result in dilated cardiomyopathy and skeletal myopathy. The most severely affected dilated cardiomyopathy patients harbor Titin truncations in the C-terminal two-thirds of the protein, suggesting that mutation position might influence disease mechanism.
Jennifer Osten et al.
The Journal of general physiology, 154(10) (2022-09-03)
The β-myosin heavy chain expressed in ventricular myocardium and the myosin heavy chain (MyHC) in slow-twitch skeletal Musculus soleus (M. soleus) type-I fibers are both encoded by MYH7. Thus, these myosin molecules are deemed equivalent. However, some reports suggested variations
Saskia Hutten et al.
Cell reports, 33(12), 108538-108538 (2020-12-29)
Nuclear import receptors, also called importins, mediate nuclear import of proteins and chaperone aggregation-prone cargoes (e.g., neurodegeneration-linked RNA-binding proteins [RBPs]) in the cytoplasm. Importins were identified as modulators of cellular toxicity elicited by arginine-rich dipeptide repeat proteins (DPRs), an aberrant
Articles
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.
Protocols
Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.
Related Content
Datasheet
Global Trade Item Number
| SKU | GTIN |
|---|---|
| S4942-200ML | 04061838256362 |
| S4942-1L | 04061838256355 |
| S4942-5L | 04061836933746 |
-
Salve Sybro ruby è utilizzabile per colorare membrane blot PVDF?
1 answer-
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service