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NUC201

Sigma-Aldrich

Nuclei Isolation Kit: Nuclei PURE Prep

sufficient for 15 nuclei preparations (~1-10×107 cells or 1g of tissue per preparation)

Sinonimo/i:

Sucrose centrifugation nuclei isolation

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.32

impiego

sufficient for 15 nuclei preparations (~1-10×107 cells or 1g of tissue per preparation)

Livello qualitativo

200

Confezionamento

pkg of 1 kit

Condizioni di stoccaggio

dry at room temperature

applicazioni

cell analysis

Attività estranea

nuclease and protease, free

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

Categorie correlate

Applicazioni

For preparation of pure nuclei and fragile nuclei from cell lines and solid tissues.

Azioni biochim/fisiol

The protocol incorporates centrifugation through a dense sucrose cushion to protect nuclei and strip away cytoplasmic contaminants. High yield has been obtained from common cell lines (Jurkat, HFN7.1, COS7, HEK293 and MDCK) and tissues (spleen and liver). These preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones, and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.
The protocol incorporates centrifugation through a dense sucrose cushion to protect nuclei and strip away cytoplasmic contaminants. The sucrose concentration that is suitable for a particular cell type is determined empirically by the user. The sucrose concentrate and sucrose cushion buffer give the user flexibility to modify the density of the sucrose cushion as appropriate. High yield has been obtained from common cell lines (Jurkat, HFN7.1, COS7, HEK293 and MDCK) and tissues (spleen and liver). These preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones, and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

Solo come componenti del kit

N° Catalogo
Descrizione
  • Nuclei PURE Lysis Buffer 180 mL

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

08168

Timestrip Plus™ 8 °C

Pittogrammi

CorrosionEnvironment
GHS05,GHS09

Avvertenze

Danger

Indicazioni di pericolo

H315,H318,H410

Classi di pericolo

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2

Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Analysis of nuclear RNA, Chapter 14.
Robert E. Farrell, Jr., ed.
RNA Methodologies: A Laboratory Guide for Isolation and Characterization, 235-263 (1993)
Nuclei from rat liver: isolation method that combines purity with high yield.
G Blobel et al.
Science (New York, N.Y.), 154(3757), 1662-1665 (1966-12-30)
Tyler G Ekins et al.
eLife, 9 (2020-11-06)
Type I lissencephaly is a neuronal migration disorder caused by haploinsuffiency of the PAFAH1B1 (mouse: Pafah1b1) gene and is characterized by brain malformation, developmental delays, and epilepsy. Here, we investigate the impact of Pafah1b1 mutation on the cellular migration, morphophysiology
Michaela Patterson et al.
Nature genetics, 49(9), 1346-1353 (2017-08-08)
Adult mammalian cardiomyocyte regeneration after injury is thought to be minimal. Mononuclear diploid cardiomyocytes (MNDCMs), a relatively small subpopulation in the adult heart, may account for the observed degree of regeneration, but this has not been tested. We surveyed 120
Identification of newly transcribed RNA.
Greenberg, M.E., and Bender, T.P., et al. et al.
Current Protocols in Molecular Biology, 4-4 (1987)
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Articoli

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

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