2-Mercaptoethanol is compatible with a wide range of common aqueous buffers, such as Laemmli sample buffer, phosphate-buffered saline, or Tris buffer. Certain buffers, such as Tris, can cause oxidation of 2-mercaptoethanol. In these situations, preparation of the solution is recommended just before use.
M3148
2-Mercaptoethanol
Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
Synonym(s):
β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 25 mL | Available to ship TODAYfromMILWAUKEE | $30.10 | |
| 100 mL | Available to ship TODAYfromMILWAUKEE | $90.80 | |
| 250 mL | Available to ship TODAYfromMILWAUKEE | $128.00 | |
| 500 mL | Available to ship TODAYfromMILWAUKEE | $235.00 |
About This Item
Linear Formula:
HSCH2CH2OH
CAS Number:
Molecular Weight:
78.13
UNSPSC Code:
12161504
NACRES:
NA.31
PubChem Substance ID:
EC Number:
200-464-6
Beilstein/REAXYS Number:
773648
MDL number:
grade
Molecular Biology
Quality Level
vapor density
2.69 (vs air)
vapor pressure
1 mmHg ( 20 °C)
product line
BioReagent
assay
99% (GC/titration)
form
liquid
expl. lim.
18 %
reaction suitability
reagent type: reductant
concentration
14.3 M (pure liquid)
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Related Categories
1 of 1
This Item | |||
|---|---|---|---|
| grade Molecular Biology | grade Molecular Biology | grade derivatization grade (HPLC) | grade certified reference material, TraceCERT® |
| technique(s) cell culture | mammalian: suitable, electrophoresis: suitable | technique(s) RNA extraction: suitable, protein extraction: suitable | technique(s) HPLC: suitable | technique(s) - |
| assay 99% (GC/titration) | assay ≥99.0% (GC) | assay ≥99.0% (GC) | assay - |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 300 |
| product line BioReagent | product line BioUltra | product line - | product line TraceCERT® |
| solubility H2O: soluble 1 mL/mL | solubility H2O: 0.5 M at 20 °C, clear, colorless | solubility - | solubility - |
Application
BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.
Reducing agent
β-mercaptoethanol or 2-mercaptoethanol has been used:
β-mercaptoethanol or 2-mercaptoethanol has been used:
- as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture DT40 cells (chicken B cell line)
- to lyse NuLi-1 (Normal Lung, University of Iowa-1) cells
- to trypsinize mouse embryonic stem cells[1]
signalword
Danger
Hazard Classifications
Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral
target_organs
Liver,Heart
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
flash_point_f
165.2 °F - closed cup
flash_point_c
74 °C - closed cup
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Protocols
Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.
This page shows and discusses three protocols for stripping and reprobing a western blot membrane.
MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.
Protocolo del ensayo con MTT para medir la viabilidad, la proliferación y la citotoxicidad celulares. Instrucciones para la preparación de los reactivos MTT y ejemplos de aplicaciones.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| M3148-25ML | 04061838258496 |
| M3148-.9ML | 04061838243348 |
| M3148-2ML | 04061838243355 |
| M3148-.15ML | 04061838080097 |
| M3148-250ML | 04061838258489 |
| M3148-500ML | 04061834046424 |
| M3148-100ML | 04061838258472 |
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In which buffer or solvent should 2-Mercaptoethanol be diluted for working solution preparation?
1 answer-
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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I’ve purchased 25mL bottle and want to use this in my cell culture media at concentration of 50mM. What is the concentration in the 25mL bottle and how to make this to 50mM concentration?
1 answer-
This product is a neat or pure material. The concentration is considered to be 100%. The calculated molarity is 14.3 M as reported in the Properties section of the product page.
To prepare a 50 mM solution, please mix 0.391 g of M3148 with 100 mL solvent. 0.391 g / 78.13 g/mol / 0.1 L = 0.05 = 50 mM.Helpful?
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Hi, What is the concentration of 2-mercaptoethanol in the 100mL bottle?
1 answer-
This product is a neat or pure material. The concentration is considered to be 100%. The calculated molarity is 14.3 M as reported in the Properties section of the product page.
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Hi is this sterile?
1 answer-
This product is not sterile, but can be used in cell culture applications and is DNase, RNase, and protease free.
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