This product is expressed in HEK293 cells while other product options are expressed in E. coli. Unfortunately, there is no GM-CSF from Jurkat currently available.
H5666
Granulocyte-Macrophage Colony-Stimulating Factor human
HumanKine®, ≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
Sinonimo/i:
GM-CSF human
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10 μG
526,00 €
5 X 10 μG
1.180,00 €
526,00 €
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Informazioni su questo articolo
UNSPSC Code:
12352202
NACRES:
NA.77
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Permettici di aiutartiNome del prodotto
Granulocyte-Macrophage Colony-Stimulating Factor human, GM-CSF, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
biological source
human
recombinant
expressed in HEK 293 cells
assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
≤5 ng/mL EC50
quality
endotoxin tested
mol wt
dimer 15-36 kDa (glycosylated)
packaging
pkg of 5X10 μg
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/mg
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
human ... CSF2(1437)
Cerchi prodotti simili? Visita Guida al confronto tra prodotti
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Questo articolo | SRP3050 | G0282 | G5035 |
|---|---|---|---|
| biological source human | biological source human | biological source mouse | biological source human |
| recombinant expressed in HEK 293 cells | recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli |
| assay ≥95% (SDS-PAGE) | assay ≥98% (SDS-PAGE) | assay ≥97% (SDS-PAGE) | assay ≥98% (SDS-PAGE) |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized | form lyophilized powder | form lyophilized powder |
Analysis Note
The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
Application
Granulocyte-Macrophage Colony-Stimulating Factor human has been used as a colony-stimulating cytokine in human acute myeloid leukemia cell line (OCI-M2) to test its effect on 5-azacitidin (AZA)-mediated DNA demethylation of upstream regulatory element (URE).[1]
Biochem/physiol Actions
Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is an immune modulator and proinflammatory cytokine. It binds to the heteromeric cell-surface receptors present in the immune cells like granulocytes, endothelial cells and macrophages.[2] GM-CSF is implicated in inflammation associated disorder of the lung and renal system.[2]
Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.
Disclaimer
Although human and mouse GM-CSF share 54% amino acid sequence homology, their biological actions are species-specific and exhibit no cross-species reactivity.
General description
Granulocyte-Macrophage Colony-Stimulating Factor is mapped to human chromosome 5q21-q32.[3]
Physical form
Lyophilized from a 0.2 μm filtered solution of 1x PBS.
Preparation Note
HumanKine GM-CSF is expressed as a 15-36 kDa glycosylated monomer in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications. Although human and mouse GM-CSF share 54% amino acid sequence homology, their biological actions are species-specific and exhibit no cross-species reactivity.
Legal Information
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
Classe di stoccaggio
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
K Huebner et al.
Science (New York, N.Y.), 230(4731), 1282-1285 (1985-12-13)
Human granulocyte-macrophage colony-stimulating factor (GM-CSF) is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. A full-length complementary DNA clone encoding human GM-CSF was used as a probe to screen a human
Yufang Shi et al.
Cell research, 16(2), 126-133 (2006-02-14)
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is an important hematopoietic growth factor and immune modulator. GM-CSF also has profound effects on the functional activities of various circulating leukocytes. It is produced by a variety of cell types including T cells, macrophages, endothelial
Julie M Vose et al.
Leukemia & lymphoma, 50(9), 1412-1421 (2009-07-16)
In autologous stem cell transplantation, mobilized peripheral blood has replaced the bone marrow as the preferred source of hematopoietic stem cells (HSCs). Because HSCs normally exist in the blood in very low numbers, the use of agents to "mobilize" HSCs
Stephanie S Watowich et al.
Immunological reviews, 238(1), 76-92 (2010-10-26)
Understanding the diversification of dendritic cell (DC) lineages is one of the last frontiers in mapping the developmental hierarchy of the hematopoietic system. DCs are a vital link between the innate and adaptive immune responses; thus, elucidating their developmental pathways
N Curik et al.
Leukemia, 26(8), 1804-1811 (2012-02-22)
Epigenetic 5-azacitidine (AZA) therapy of high-risk myelodysplastic syndromes (MDS) and acute myelogenous leukemia (AML) represents a promising, albeit not fully understood, approach. Hematopoietic transcription factor PU.1 is dynamically regulated by upstream regulatory element (URE), whose deletion causes downregulation of PU.1
Articoli
Read article on hematopoietic cytokines and hematopoiesis
Contenuto correlato
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Can we find GM-CSF human expressed in Jurkat cells?
1 risposta-
Utile?
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Welche Anzahl an U befindet sich pro mL?
1 risposta-
The specific activity is determined by the dose dependent stimulation of the proliferation of the human TF-1 cells (human erythroleukemic indicator cell line). This is provided as an EC50 value, meaning half the maximal effective concentration.
The EC50 for the specific lot will be found on the COA.
https://www.sigmaaldrich.com/product/sigma/h5666#product-documentationUtile?
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