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E5529

EZview™ Red Streptavidin Affinity Gel

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NACRES:
NA.56
UNSPSC Code:
41106500
Pricing and availability is not currently available.
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form

suspension

Quality Level

200

shelf life

1 yr

technique(s)

western blot: suitable

matrix

4% agarose

pH

7.2

capacity

~10 μg(biotin per ml of packed gel)

shipped in

wet ice

storage temp.

2-8°C

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EZview™ Red Streptavidin Affinity Gel

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E6406

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EZview™ Red Anti-HA Affinity Gel

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E6779

EZview™ Red Anti-HA Affinity Gel

EZview™ Red Protein G Affinity Gel

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E3403

EZview™ Red Protein G Affinity Gel

matrix

4% agarose

matrix

-

matrix

crosslinked agarose

matrix

-

capacity

~10 μg(biotin per ml of packed gel)

capacity

-

capacity

≥0.4 mg/mL, gel binding capacity (HA-tagged fusion protein)

capacity

≥8 mg/mL, gel binding capacity (rabbit IgG)

technique(s)

western blot: suitable

technique(s)

immunoprecipitation (IP): suitable, protein purification: suitable

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

form

suspension

form

-

form

-

form

liquid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

-

General description

The EZ View Red Streptavidin Affinity gel is composed of streptavidin, covalently attached to
cyanogen bromide-activated 4% agarose beads. It is designed to capture (pull-down) the biotinylated target molecules, such as proteins, peptides, antibodies, nucleic acids, lectins, receptors and ligands.

Application

Sutitable for use with immunoprecipitation, western blotting,and enzyme assays.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview™ resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

Features and Benefits

  • Increased visibility - Red color reduces risk of incidental aspiration
  • Improved recovery of target protein by reduced accidental loss
  • Higher reproducibility - More consistent yields

Physical form

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon

Legal Information

EZview is a trademark of Sigma-Aldrich Co. LLC

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000507127
0000507127
0000383624
0000348360
0000348360

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Suet Yin Sarah Fung et al.
Current biology : CB, 27(1), 78-86 (2016-12-13)
After cleavage furrow ingression during cytokinesis, nascent daughter cells remain connected by an intercellular bridge (ICB) and the midbody [1, 2]. The midbody becomes an assembly platform for ESCRT complexes that split apart the plasma membrane (PM) anchored to the
Alicia M Bostwick et al.
Biochemical and biophysical research communications, 528(3), 580-585 (2020-06-09)
Mammalian cells contain genetic information in two compartments, the nucleus and the mitochondria. Mitochondrial gene expression must be coordinated with nuclear gene expression to respond to cellular energetic needs. To gain insight into the coordination between the nucleus and mitochondria
Katarzyna M Zientara-Rytter et al.
Cells, 11(1) (2022-01-12)
Pex11, an abundant peroxisomal membrane protein (PMP), is required for division of peroxisomes and is robustly imported to peroxisomal membranes. We present a comprehensive analysis of how the Pichia pastoris Pex11 is recognized and chaperoned by Pex19, targeted to peroxisome
Iram Khan Iqbal et al.
Autophagy, 17(11), 3511-3529 (2021-01-19)
The deacetylase SIRT1 (sirtuin 1) has emerged as a major regulator of nucleocytoplasmic distribution of macroautophagy/autophagy marker MAP1LC3/LC3 (microtubule-associated protein 1 light chain 3). Activation of SIRT1 leads to the deacetylation of LC3 and its translocation from the nucleus into
Ting-Yu Chang et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 138, 111485-111485 (2021-03-20)
Aberrant alteration of epigenetic information disturbs chromatin structure and gene function, thereby facilitating cancer development. Several drugs targeting histone deacetylases (HDACs), a group of epigenetic enzymes, have been approved for treating hematologic malignancies in the clinic. However, patients who suffer
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Product Information Sheet - E5529
GC5, GC10, and Thunderbolt Competent Cells

Global Trade Item Number

SKUGTIN
E5529-1ML04061822788947
E5529-5X1ML04061822788954

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