This recombinant Albumin is extracted and purified from the seeds of Oryza sativa (rice) and not produced by culture methods. The material is considered animal component-free. The product is not tested for any residual steroid hormones native to this plant species.
A9731
Albumin human
Cellastim S™, recombinant, expressed in rice, lyophilized powder, suitable for cell culture, low endotoxin, ≥96% (SDS-PAGE)
Albumin human
Synonym(s):
Cellastim S™, rHSA, rHSA
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About This Item
Empirical Formula (Hill Notation):
C2936H4624N786O889S41
CAS Number:
Molecular Weight:
66471.53
UNSPSC Code:
12352202
eCl@ss:
42010201
NACRES:
NA.25
MDL number:
Biological source:
human
Assay:
≥96% (SDS-PAGE)
Form:
lyophilized powder
Technique(s):
cell culture | mammalian: suitable
Impurities:
≤1 EU/mg protein
Pricing and availability is not currently available.
biological source
human
Quality Level
recombinant
expressed in rice
assay
≥96% (SDS-PAGE)
form
lyophilized powder
mol wt
monomer ~67 kDa
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/mg protein
UniProt accession no.
storage temp.
2-8°C
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This Item | |||
|---|---|---|---|
| biological source human | biological source human | biological source human | biological source human |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) ELISA: suitable, tissue culture: suitable, western blot: suitable | technique(s) ELISA: suitable, tissue culture: suitable, western blot: suitable |
| assay ≥96% (SDS-PAGE) | assay ≥90% (agarose gel electrophoresis) | assay ≥99% (agarose gel electrophoresis) | assay ≥96% (agarose gel electrophoresis) |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 300 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| Gene Information human ... ALB(213) | Gene Information human ... ALB(213) | Gene Information human ... ALB(213) | Gene Information human ... ALB(213) |
Application
This recombinant variant is recommended for use in absorption, distribution, metabolism, and excretion (ADME) pharmacological research; cell culture; drug delivery research; and cryopreservation of cells.
Biochem/physiol Actions
Albumins are soluble monomeric proteins found in the body fluids and tissues of animals and in some plant seeds. Serum albumin functions as a carrier protein for steroids, fatty acids, and thyroid hormones. Serum albumins are also vital in regulating the colloidal osmotic pressures of blood.
Features and Benefits
Cellastim™ is a well defined, animal-free recombinant human albumin (rHA). Designed for cell culture applications, Cellastim is a powerful media supplement that outperforms plasma-derived human albumin, bovine serum albumin, and other sources of recombinant human albumin. Cellastim delivers consistent performance, not the variability associated with hydrolysates and extracts. Cellastim is ideally suited for use in cell culture media for Chinese Hamster Ovary (CHO), hybridoma, VERO, stem cells and primary cells.
Advantages
Advantages
- Eliminates animal-derived additives
- Safe and regulatory friendly
- Proven effective in enhancing stem cell growth in embryonic, mesenchymal, neural, cardiac, liver, kidney, keratinocyte and fibroblast stem cell lines
- Maintains stem cells in an undifferentiated state
- Eliminates coating of culture plates for adherent cell lines
- Provides well-defined and consistent stem cell medium
Analysis Note
Assayed by SDS-PAGE
Other Notes
1). Patent Rights
EP2230311
U.S. Patent No. 10618951
U.S. Patent No. 10981974
U.S. Patent No. 11492389
2). Registered Trademark Rights
U.S. Reg. 5696531
International Reg. 1456207
EP2230311
U.S. Patent No. 10618951
U.S. Patent No. 10981974
U.S. Patent No. 11492389
2). Registered Trademark Rights
U.S. Reg. 5696531
International Reg. 1456207
To view more information on Cellastim download the Cellastim Brochure, Cellastim CHO Poster, and Cellastim hybridoma Poster.
View more information on human serum albumin.
Legal Information
rHSA manufactured in the USA by InVitria US Pat 10618951, 10981974, and 11492389.
Cellastim is a trademark of InVitria
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Description
Pricing
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Wireeya Chawjiraphan et al.
MethodsX, 7, 101114-101114 (2020-11-17)
A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of
Yasuhiro Kawai et al.
PloS one, 13(5), e0196655-e0196655 (2018-05-26)
Serum albumin is the major intravascular antioxidant. Though oxidative stress plays an important role in the pathophysiology of Immunoglobulin A nephropathy (IgAN), the association between serum albumin and the progression of IgAN is not entirely understood. This retrospective cohort study
Yongjia Fan et al.
ACS biomaterials science & engineering, 3(8), 1510-1518 (2016-08-14)
The development of platforms for the expansion and directed differentiation of human pluripotent stem cells (hPSCs) in large quantities under xeno-free conditions is a key step toward the realization of envisioned stem cell-based therapies. Microcarrier bioreactors afford great surface-to-volume ratio
D Moreno et al.
Theriogenology, 84(7), 1053-1060 (2015-08-19)
The aim of this study was to develop an in vitro embryo culture medium without either fetal calf serum or BSA, using various growth factors and cytokines (GFs-CYKs; IGF-I, IGF-II, bFGF, LIF, GM-CSF, TGF-β1, and PDGF-BB), and other molecules with
Thomas J Esparza et al.
Scientific reports, 10(1), 22370-22370 (2020-12-24)
There are currently few approved effective treatments for SARS-CoV-2, the virus responsible for the COVID-19 pandemic. Nanobodies are 12-15 kDa single-domain antibody fragments that can be delivered by inhalation and are amenable to relatively inexpensive large scale production compared to other
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| A9731-10G | 04061833412275 |
| A9731-1G | 04061833412282 |
| A9731-5G | 04061833412299 |
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Is this obtained from cultures supplemented with media containing any form of steroids or animal derived components? Does it contain any form of sterioids as part of the impurities?
1 answer-
Helpful?
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"Does this HSA product contain free thiol at Cys34 (i.e., mercaptalbumin form)?"
1 answer-
Unfortunately, we do not separately verify the free thiol % for this product. Therefore, we are unable to provide any information. We apologize for not being able to assist you.
Helpful?
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Hello, does this construct include any affinity tags? Does it contain glycosylation?
1 answer-
A9731 is free from affinity tags and it is glysylated. We have no information on glycosylations sites.
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