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Xanthine Oxidase from bovine milk

Name: Xanthine Oxidase from bovine milk
Brand: SIGMA
Price: 69.8 USD

Grade I, ammonium sulfate suspension, ≥0.4 units/mg protein

Synonym(s):

XOD, Xanthine:oxygen oxidoreductase

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Pack Size	Availability	Price
5 units	Check Cart for Availability	$69.80
25 units	Check Cart for Availability	$202.00
50 units	Check Cart for Availability	$312.00

About This Item

CAS Number:

9002-17-9

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-657-6

MDL number:

MFCD00082145

EC Number:

1.17.3.2.

Specific activity:

≥0.4 units/mg protein

$69.80

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type

Grade I

Quality Level

200

form

ammonium sulfate suspension

specific activity

≥0.4 units/mg protein

foreign activity

uricase ≤0.5%

shipped in

wet ice

storage temp.

2-8°C

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This Item	X4500	X4875	10110434001
Sigma-Aldrich X1875 Xanthine Oxidase from bovine milk	Sigma-Aldrich X4500 Xanthine Oxidase from bovine milk	Sigma-Aldrich X4875 Xanthine Oxidase from bovine milk	Roche 10110434001 Xanthine Oxidase (XOD)
specific activity ≥0.4 units/mg protein	specific activity ≥0.8 units/mg protein	specific activity ≥0.1 units/mg protein	specific activity ~1 units/mg protein (At 25 °C with xanthine as the substrate.)
form ammonium sulfate suspension	form ammonium sulfate suspension	form ammonium sulfate suspension	form suspension
shipped in wet ice	shipped in -	shipped in -	shipped in wet ice
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level -
foreign activity uricase ≤0.5%	foreign activity uricase ≤0.1%	foreign activity -	foreign activity -

General description

Xanthine Oxidase (XOD) is a metal flavoprotein. It has flavin adenine dinucleotide (FAD), molybdenum and iron in the ratio 2:2:8. This homodimer has a molecule weight of 290kDa. It is a member of the molybdenum-protein family. This enzyme consists of two separated substrate-binding sites.

Formerly E.C. 1.1.3.22

Application

Xanthine Oxidase from bovine milk has been used:

in the preparation of xanthine oxidase (XO) solution for 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO)-spin trapping assay
in in vitro XO assay for screening Vietnamese medicinal plants for XO inhibitory activity
as a standard to determine XO activity
as a standard to test the synergistic effect of docosahexaenoic acid (DHA)

Biochem/physiol Actions

Hydroxylation of hypoxanthine to xanthine and xanthine to uric acid is catalyzed by xanthinebb oxidase (XO) enzyme.

Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species.

Xanthine oxidase was shown to be involved in the reduction of cytochrome c by the generation of superoxide anions following the oxidation of xanthine. These free radicals are responsible for reducing cytochrome c. [1]

Physical form

Suspension in 2.3 M (NH₄)₂SO₄ containing 1 mM sodium salicylate

Analysis Note

Protein determined by biuret

Other Notes

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P304 + P340 + P312 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Methods of Enzymatic Analysis (2012)

Antioxidant properties of propofol and erythropoietin after closed head injury in rats

Ozturk E, et al.

Progress in Neuro-Psychopharmacology & Biological Psychiatry, 29, 922-927 (2005)

Xanthine oxidase inhibitors from Archidendron clypearia (Jack.) I.C. Nielsen: Results from systematic screening of Vietnamese medicinal plants

Duong NT, et al.

Asian Pacific Journal of Tropical Medicine, 10(6), 549-556 (2017)

Protein-bound molybdenum cofactor is bioavailable and rescues molybdenum cofactor-deficient C. elegans.

Kurt Warnhoff et al.

Genes & development, 35(3-4), 212-217 (2021-01-16)

The molybdenum cofactor (Moco) is a 520-Da prosthetic group that is synthesized in all domains of life. In animals, four oxidases (among them sulfite oxidase) use Moco as a prosthetic group. Moco is essential in animals; humans with mutations in

The reduction of cytochrome c by milk xanthine oxidase.

J M McCord et al.

The Journal of biological chemistry, 243(21), 5753-5760 (1968-11-10)

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Global Trade Item Number

SKU	GTIN
X1875-5UN	04061838258366
X1875-25UN	04061838258342
X1875-50UN	04061838258359

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