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SRE0095

Fast β-Glucuronidase, Recombinant

from limpets (Patella Vulgata), aqueous solution

Synonym(s):

β-Glucuronidase from limpets (Patella vulgata), β-D-Glucuronide glucuronosohydrolase

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Pack SizeSKUAvailabilityPrice
10 mL

Available to ship TODAYfromMILWAUKEE

$476.00
25 mL

Available to ship TODAYfromMILWAUKEE

$1,060.00
50 mL

Available to ship TODAYfromMILWAUKEE

$2,150.00

About This Item

CAS Number:
9001-45-0
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
3.2.1.31 (BRENDA, IUBMB)
MDL number:
MFCD00130629
Recombinant:
expressed in proprietary host
Concentration:
300000-400000 units/mL

$476.00

Available to ship TODAYDetails

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recombinant

expressed in proprietary host

Quality Level

200

form

aqueous solution

concentration

300000-400000 units/mL

shipped in

ambient

storage temp.

2-8°C

Application

New Technical Article Comparing Performance of Different Enzymes
Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
More on the suitability of this product for hydrolysis of different drug-glucuronide substrates is available at this page.
The enzyme from patella vulgata is reported to be more effective in hydrolyzing opioid-glucuronides than the Helix pomatia, bovine liver and E. coli enzymes

Features and Benefits

Formulated with a higher enzyme concentration for applications requiring ultra-fast hydrolysis rates. Recombinantly expressed and purified to eliminate MAM esterase activity (6-Monoacetylmorphine→morphine). Representative analysis at high enzyme titer (50 un/μL β-glucuronidase) was less than 1% conversion of 6-MAM to morphine after 4 hours at 60 °C.

Physical form

Supplied as a solution in 100mM potassium acetate pH 5.2. It is free of detergents, carbohydrates or any other components that may interfere with sample preparation and analysis.

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).

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This Item
G8162G8420G8295
Fast β-Glucuronidase, Recombinant from limpets (Patella Vulgata), aqueous solution

Sigma-Aldrich

SRE0095

Fast β-Glucuronidase, Recombinant

β-Glucuronidase from Escherichia coli aqueous glycerol solution, ≥5,000,000 units/g protein, pH 6.8 (biuret)

Sigma-Aldrich

G8162

β-Glucuronidase from Escherichia coli

β-Glucuronidase from Escherichia coli ≥20,000 units/mg protein, recombinant, expressed in E. coli overproducing strain, lyophilized powder

Sigma-Aldrich

G8420

β-Glucuronidase from Escherichia coli

β-Glucuronidase from Escherichia coli ≥10,000,000 units/g protein (30 min assay), recombinant, expressed in E. coli overproducing strain, lyophilized powder

Sigma-Aldrich

G8295

β-Glucuronidase from Escherichia coli

recombinant

expressed in proprietary host

recombinant

-

recombinant

expressed in E. coli overproducing strain

recombinant

expressed in E. coli overproducing strain

concentration

300000-400000 units/mL

concentration

-

concentration

-

concentration

-

form

aqueous solution

form

aqueous glycerol solution

form

lyophilized powder

form

lyophilized powder

shipped in

ambient

shipped in

wet ice

shipped in

-

shipped in

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

300

Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000481190
0000481190
0000374450
0000363732
0000239476

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Articles

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Related Content

Global Trade Item Number

SKUGTIN
SRE0095-1ML04061834743316
SRE0095-50ML04061831160079
SRE0095-25ML04061831160062
SRE0095-10ML04061831160055

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