H9164
Anti-acetyl-Histone H4 (Ac-Lys16) antibody produced in rabbit
~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-H4K16ac
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About This Item
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
WB
Citations:
19
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~12 kDa
species reactivity
human
concentration
~1.5 mg/mL
technique(s)
western blot: 1-2 μg/mL using HL60 cell lysates
General description
The building block of chromatin is the nucleosome which is comprised of an octamer of core histones (H2A, H2B, H3, and H4) around which 147 bp of DNA are wrapped. Histones H3 and H4 are the predominant histones subjected to covalent modifications.[1][2] Acetylation of histone H4 on Lys16 (H4-K16Ac) is a prevalent modification that plays a role in transcriptional activation by regulating higher-order chromatin structure and protein interactions.[3][4]
acetyl-Histone H4 (Ac-Lys16) is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.
Immunogen
synthetic peptide corresponding to amino acids 9-18 (Ac-Lys16) of human histone H4, conjugated to KLH.
Application
Anti-acetyl-Histone H4 (Ac-Lys16) antibody produced in rabbit is suitable for western blot at a concentration of 1-2μg/mL using HL60 cell lysates.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Immunohistochemistry (1 paper)
Chromatin immunoprecipitation (1 paper)
Immunohistochemistry (1 paper)
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Noriyuki Suka et al.
Nature genetics, 32(3), 378-383 (2002-10-16)
The Sir3 protein helps form telomeric heterochromatin by interacting with hypoacetylated histone H4 lysine 16 (H4-Lys16). The molecular nature of the heterochromatin boundary is still unknown. Here we show that the MYST-like acetyltransferase Sas2p is required for the acetylation (Ac)
Yong Wang et al.
Journal of experimental & clinical cancer research : CR, 32, 8-8 (2013-02-12)
MYST1 (also known as hMOF), a member of the MYST family of histone acetyltransferases (HATs) as an epigenetic mark of active genes, is mainly responsible for histone H4K16 acetylation in the cells. Recent studies have shown that the abnormal gene
Michael Shogren-Knaak et al.
Science (New York, N.Y.), 311(5762), 844-847 (2006-02-14)
Acetylation of histone H4 on lysine 16 (H4-K16Ac) is a prevalent and reversible posttranslational chromatin modification in eukaryotes. To characterize the structural and functional role of this mark, we used a native chemical ligation strategy to generate histone H4 that
Yamini Sabherwal et al.
Oncology reports, 30(4), 1985-1988 (2013-08-08)
The importance of epigenetic alterations such as DNA methylation, histone modification and nucleosome remodeling in breast cancer is well established. Epigenetic alterations are reversible, and much research has been focused on understanding these alterations with the aim of developing effective
Da Liu et al.
PloS one, 10(10), e0141014-e0141014 (2015-10-17)
Histone post-translational modification heritably regulates gene expression involved in most cellular biological processes. Experimental studies suggest that alteration of histone modifications affects gene expression by changing chromatin structure, causing various cellular responses to environmental influences. Arsenic (As), a naturally occurring
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